Schmid, A. and Kopp, A. and Aslanidis, C. and Wabitsch, M. and Mueller, M. and Schaeffler, A. (2013) Regulation and Function of C1Q/TNF-related Protein-5 (CTRP-5) in the Context of Adipocyte Biology. EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES, 121 (5). pp. 310-317. ISSN 0947-7349,
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Background and aim: The C1q/TNF-related protein (CTRP) family represents a growing family of adiponectin paralogous proteins. CTRP-5 was detected in adipose tissue of mice and plays a role in the context of the metabolic syndrome. It was the aim to investigate the detailed expression profile of CTRP-5 in a variety of adipocytic cells and to determine whether CTRP-5 circulates in human serum. Moreover, regulation and function of CTRP-5 was studied in the context of adipocyte biology. Material and methods: CTRP-5 serum levels were measured in 50 healthy subjects by ELISA. Genotype analysis was performed by direct sequencing in 200 probands. CTRP-5 mRNA and protein expression was analyzed by RT-PCR, real-time RT-PCR and Western blot. Recombinant CTRP-5 and fatty acids were used for stimulation experiments in 3T3-L1 adipocytes. siRNA-mediated knockdown of CTRP-3 was performed during adipocyte differentiation. Results: CTRP-5 mRNA and protein was strongly expressed in a wide variety of human and murine adipocytic cells and was induced during adipocyte differentiation. Saturated fatty acids increased CTRP-5 expression in adipocytes. siRNA-mediated cellular knockdown of CTRP-3 in adipocytes resulted in an upregulation of CTRP-5 expression. CTRP-5 inhibited the release of resistin and adiponectin dose-dependently. CTRP-5 circulates abundantly in human sera with a broad interindividual variation. The SNP 1014 T/A was not associated with type 2 diabetes mellitus in 200 Caucasian probands. Conclusions: CTRP-5 might be a novel adipokine that circulates abundantly in human sera. CTRP-5 is functionally involved in adipocyte biology and there might exist a counter-regulatory connection with its family member, CTRP-3.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; ADIPOSE-TISSUE; INNATE IMMUNITY; FAMILY; DIFFERENTIATION; ADIPONECTIN; METABOLISM; EXPRESSION; GLUCOSE; C1Q; CTRP-5; adipocyte; adipokine; adipose tissue; single nucleotide polymorphism |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2020 12:00 |
| Last Modified: | 09 Apr 2020 12:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16711 |
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