Boxhammer, V. and Li, Y. F. and Koeritzer, J. and Shimizu, T. and Maisch, T. and Thomas, H. M. and Schlegel, J. and Morfill, G. E. and Zimmermann, J. L. (2013) Investigation of the mutagenic potential of cold atmospheric plasma at bactericidal dosages. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 753 (1). pp. 23-28. ISSN 1383-5718,
Full text not available from this repository. (Request a copy)Abstract
In the past few years, cold atmospheric plasma (CAP) has evolved into a new tool in the fight against nosocomial infections and antibiotic-resistant microorganisms. The products generated by the plasma-electrons, ions, reactive species and UV light-represent a 'lethal cocktail' for different kinds of pathogen, which opens up possible applications in hygiene and medicine. Nevertheless, to ensure the safe usage of CAP on skin (e.g., to treat wounds or skin diseases) several pre-clinical in vitro studies have to be performed before implementing clinical trials on humans. In the study presented here, inactivation experiments with Escherichia coli were carried out to identify the necessary plasma dosage for a 5 log reduction: with a small hand-held battery-operated CAP device, these disinfection properties were achieved after application during 30 s. This and higher plasma dosages were then used to analyze the mutagenicity induced in V79 Chinese hamster cells-to furthermore define a 'safe application window'-with the HPRT (hypoxanthineguanine phosphoribosyl transferase) mutation assay. The results show that a CAP treatment of up to 240s and repeated treatments of 30 s every 12h did not induce mutagenicity at the Hprt locus beyond naturally occurring spontaneous mutations. (C) 2013 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHINESE-HAMSTER CELLS; TISSUE-TOLERABLE PLASMA; RANDOMIZED CONTROLLED-TRIAL; ETHYL METHANESULFONATE; ELECTROMAGNETIC-FIELDS; ULTRAVIOLET-RADIATION; MOLECULAR DOSIMETRY; LIMITING EXPOSURE; ESCHERICHIA-COLI; CHRONIC WOUNDS; Cold atmospheric plasma; Mutagenicity; Hprt; V79 cells; Bacteria; Plasma medicine |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Apr 2020 07:49 |
| Last Modified: | 16 Apr 2020 07:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16797 |
Actions (login required)
 |
View Item |
