Zaparty, Melanie and Hagemann, Anna and Braesen, Christopher and Hensel, Reinhard and Lupas, Andrei N. and Brinkmann, Henner and Siebers, Bettina (2013) The First Prokaryotic Trehalose Synthase Complex Identified in the Hyperthermophilic Crenarchaeon Thermoproteus tenax. PLOS ONE, 8 (4): e61354. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
The role of the disaccharide trehalose, its biosynthesis pathways and their regulation in Archaea are still ambiguous. In Thermoproteus tenax a fused trehalose-6-phosphate synthase/phosphatase (TPSP), consisting of an N-terminal trehalose-6-phosphate synthase (TPS) and a C-terminal trehalose-6-phosphate phosphatase (TPP) domain, was identified. The tpsp gene is organized in an operon with a putative glycosyltransferase (GT) and a putative mechanosensitive channel (MSC). The T. tenax TPSP exhibits high phosphatase activity, but requires activation by the co-expressed GT for bifunctional synthase-phosphatase activity. The GT mediated activation of TPS activity relies on the fusion of both, TPS and TPP domain, in the TPSP enzyme. Activation is mediated by complex-formation in vivo as indicated by yeast two-hybrid and crude extract analysis. In combination with first evidence for MSC activity the results suggest a sophisticated stress response involving TPSP, GT and MSC in T. tenax and probably in other Thermoproteales species. The monophyletic prokaryotic TPSP proteins likely originated via a single fusion event in the Bacteroidetes with subsequent horizontal gene transfers to other Bacteria and Archaea. Furthermore, evidence for the origin of eukaryotic TPSP fusions via HGT from prokaryotes and therefore a monophyletic origin of eukaryotic and prokaryotic fused TPSPs is presented. This is the first report of a prokaryotic, archaeal trehalose synthase complex exhibiting a much more simple composition than the eukaryotic complex described in yeast. Thus, complex formation and a complex-associated regulatory potential might represent a more general feature of trehalose synthesizing proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | YEAST TREHALOSE-6-PHOSPHATE SYNTHASE; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; MOLECULAR CHARACTERIZATION; PHOSPHATASE COMPLEX; GENE-EXPRESSION; METABOLISM; PROTEIN; ARCHAEBACTERIA; ACCUMULATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Apr 2020 08:06 |
| Last Modified: | 16 Apr 2020 08:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16804 |
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