Sekula, Peggy and Dettmer, Katja and Vogl, Franziska C. and Gronwald, Wolfram and Ellmann, Lisa and Mohney, Robert P. and Eckardt, Kai-Uwe and Suhre, Karsten and Kastenmuller, Gabi and Oefner, Peter J. and Kottgen, Anna (2017) From Discovery to Translation: Characterization of C-Mannosyltryptophan and Pseudouridine as Markers of Kidney Function. SCIENTIFIC REPORTS, 7: 17400. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Using a non-targeted metabolomics platform, we recently identified C-mannosyltryptophan and pseudouridine as non-traditional kidney function markers. The aims of this study were to obtain absolute concentrations of both metabolites in blood and urine from individuals with and without CKD to provide reference ranges and to assess their fractional excretions (FE), and to assess the agreement with their non-targeted counterparts. In individuals without/with CKD, mean plasma and urine concentrations for C-mannosyltryptophan were 0.26/0.72 mu mol/L and 3.39/4.30 mu mol/mmol creatinine, respectively. The respective concentrations for pseudouridine were 2.89/5.67 mu mol/L and 39.7/33.9 mu mol/mmol creatinine. Median (25th, 75th percentiles) FEs were 70.8% (65.6%, 77.8%) for C-mannosyltryptophan and 76.0% (68.6%, 82.4%) for pseudouridine, indicating partial net reabsorption. Association analyses validated reported associations between single metabolites and eGFR. Targeted measurements of both metabolites agreed well with the non-targeted measurements, especially in urine. Agreement for composite nephrological measures FE and urinary metabolite-to-creatinine ratio was lower, but could be improved by replacing non-targeted creatinine measurements with a standard clinical creatinine test. In summary, targeted quantification and additional characterization in relevant populations are necessary steps in the translation of non-traditional biomarkers in nephrology from non-targeted discovery to clinical application.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLOMERULAR-FILTRATION-RATE; GENERAL-POPULATION; RENAL-FUNCTION; WIDE ASSOCIATION; DISEASE GCKD; METABOLOMICS; SERUM; PREDICTION; BURDEN; COHORT; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:18 |
| Last Modified: | 25 Feb 2019 14:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1690 |
Actions (login required)
 |
View Item |
