Limm, Katharina and Ott, Corinna and Wallner, Susanne and Mueller, Daniel W. and Oefner, Peter and Hellerbrand, Claus and Bosserhoff, Anja-Katrin (2013) Deregulation of protein methylation in melanoma. EUROPEAN JOURNAL OF CANCER, 49 (6). pp. 1305-1313. ISSN 0959-8049, 1879-0852
Full text not available from this repository. (Request a copy)Abstract
Loss of methylthioadenosine phosphorylase (MTAP) expression and a concomitant accumulation of 5'-methyl-thioadenosine (MTA) characterise several tumour entities including malignant melanoma. MTA affects cellular signalling, proliferation and migration not only of cancer but also surrounding cells including lymphocytes and stromal fibroblasts. The mode of action of MTA is still not known. Interestingly, MTA is a known potent inhibitor of protein arginine methyltransferases (PRMTs) and is used as a tool in studying activity and impact of PRMTs. This study aimed at analysing PRMTs in melanoma and the potential impact of MTA on tumourigenesis. Our findings demonstrate that expression of PRMT4/CARM1 and PRMT6 is deregulated in melanoma, whereas expression of the remaining PRMTs stays unchanged. General PRMT activity and, consequently, symmetric and asymmetric protein methylation are reduced significantly in melanoma cells and tissues. This is due to a loss of MTAP expression and accumulation of MTA. Reduction of protein methylation by MTA affects cell signalling and leads, for example, to an activation of extracellular signal-regulated kinase (ERK) activity. The effects of endogeneous MTA on PRMTs as presented in this study can strongly support the migratory and invasive phenotype of melanoma cells. (C) 2012 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARGININE METHYLTRANSFERASE 5; PHOSPHORYLASE MTAP EXPRESSION; METHYLTHIOADENOSINE PHOSPHORYLASE; MALIGNANT-MELANOMA; DOWN-REGULATION; HEPATOCELLULAR-CARCINOMA; GENE-EXPRESSION; GROWTH; CARM1; PROGRESSION; PRMT; Methylthioadenosine; Melanoma; MAPK/ERK signalling; Protein arginine methylation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Apr 2020 05:05 |
| Last Modified: | 20 Apr 2020 05:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16912 |
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