Wanninger, Josef and Liebisch, Gerhard and Schmitz, Gerd and Bauer, Sabrina and Eisinger, Kristina and Neumeier, Markus and Ouchi, Noriyuki and Walsh, Kenneth and Buechler, Christa (2013) Lipidomic analysis of the liver identifies changes of major and minor lipid species in adiponectin deficient mice. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 94 (2). pp. 412-417. ISSN 0014-4800,
Full text not available from this repository. (Request a copy)Abstract
Adiponectin protects from hepatic fat storage but adiponectin deficient mice (APN-/-) fed a standard chow do not develop liver steatosis. This indicates that other pathways might be activated to compensate for adiponectin deficiency. An unbiased and comprehensive screen was performed to identify hepatic alterations of lipid classes in these mice. APN-/- mice had decreased hepatic cholesteryl esters while active SREBP2 and systemic total cholesterol were not altered. Upregulation of cytochromes for bile acid synthesis suggests enhanced biliary cholesterol excretion. Analysis of 37 individual fatty acid species showed reduced stearate whereas total fatty acids were not altered. Total amount of triglycerides and phospholipids were equally abundant. A selective increase of monounsaturated phosphatidylcholine and phosphatidylethanolamine which positively correlate with hepatic and systemic triglycerides with the latter being elevated in APN-/- mice, was identified. Stearoyl-CoA desaturase 1 (SCD1) is involved in the synthesis of monounsaturated fatty acids and despite higher mRNA expression enzyme activity was not enhanced. Glucosylceramide postulated to contribute to liver damage was decreased. This study demonstrates that adiponectin deficiency is associated with hepatic changes in lipid classes in mice fed a standard chow which may protect from liver steatosis. (C) 2012 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HIGH-THROUGHPUT QUANTIFICATION; SPECTROMETRY ESI-MS/MS; INSULIN-RESISTANCE; HUMAN HEPATOCYTES; NONALCOHOLIC STEATOHEPATITIS; LACKING ADIPONECTIN; BETA-OXIDATION; KNOCKOUT MICE; PHOSPHATIDYLCHOLINE; DESATURASES; Lipid profiling; Liver; Adiponectin deficiency; Hepatic gene expression |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Apr 2020 07:16 |
| Last Modified: | 20 Apr 2020 07:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16931 |
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