Thomas, Anja and Klein, Matthias S. and Stevens, Axel P. and Reinders, Yvonne and Hellerbrand, Claus and Dettmer, Katja and Gronwald, Wolfram and Oefner, Peter J. and Reinders, Joerg (2013) Changes in the hepatic mitochondrial and membrane proteome in mice fed a non-alcoholic steatohepatitis inducing diet. JOURNAL OF PROTEOMICS, 80. pp. 107-122. ISSN 1874-3919, 1876-7737
Full text not available from this repository. (Request a copy)Abstract
Non-alcoholic steatohepatitis (NASH) accounts for a large proportion of cryptic cirrhosis in the Western societies. Nevertheless, we lack a deeper understanding of the underlying pathomolecular processes, particularly those preceding hepatic inflammation and fibrosis. In order to gain novel insights into early NASH-development from the first appearance of proteomic alterations to the onset of hepatic inflammation and fibrosis, we conducted a time-course analysis of proteomic changes in liver mitochondria and membrane-enriched fractions of female C57Bl/6N mice fed either a mere steatosis or NASH inducing diet. This data was complemented by quantitative measurements of hepatic glycerol-containing lipids, cholesterol and intermediates of the methionine cycle. Aside from energy metabolism and stress response proteins, enzymes of the urea cycle and methionine metabolism were found regulated. Alterations in the methionine cycle occur early in disease progression preceding molecular signs of inflammation. Proteins that hold particular promise in the early distinction between benign steatosis and NASH are methyl-transferase Mettl7b, the glycoprotein basigin and the microsomal glutathione-transferase. (C) 2013 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY LIVER-DISEASE; IRON OVERLOAD; HEPATOCELLULAR-CARCINOMA; INCREASED EXPRESSION; OXIDATIVE STRESS; UREA CYCLE; RAT-LIVER; CHOLESTEROL; METABOLISM; METHIONINE; Non-alcoholic steatohepatitis; Differential two-dimensional gel electrophoresis; Stable isotope labeling; Proteomics; Metabolomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Apr 2020 05:19 |
| Last Modified: | 23 Apr 2020 05:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16967 |
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