Matysik, Silke and Schmitz, Gerd (2013) Application of gas chromatography-triple quadrupole mass spectrometry to the determination of sterol components in biological samples in consideration of the ionization mode. BIOCHIMIE, 95 (3). pp. 489-495. ISSN 0300-9084, 1638-6183
Full text not available from this repository. (Request a copy)Abstract
The hyphenation of gas chromatography (GC) and triple quadrupole mass spectrometry is a promising approach to increase sensitivity and selectivity as compared to single quad mass spectrometry. We present in this paper the application of GC-triple quadrupole mass spectrometry for determination of sterol components in biological samples. Due to the fact that sterols are quite small molecules an appropriate ionization mode has to be found for advantageous exploitation of the triple quad function. Electron ionization (El), positive and negative chemical ionization (PCI, NCI) have been tested regarding sensitivity improvement in oxysterol and bile acid analysis in plasma samples. Target analytes were 24-, 25- and 27-hydroxycholesterol, 7 beta-hydroxycholesterol, 7-ketocholesterol, 3 beta,5 alpha,6 beta-cholestanetriol, cholic acid, chenodeoxycholic acid, deoxycholic acid and lithocholic acid. In contrast to bile acids, oxysterols could be analyzed with the highest degree of sensitivity by application of PCI in multiple reaction monitoring mode whereas 7 beta-hydroxycholesterol and 7-ketocholesterol showed even better results with NCI. (C) 2012 Elsevier Masson SAS. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BILE-ACID METABOLISM; CHOLESTEROL HOMEOSTASIS; OXIDATION-PRODUCTS; OXYSTEROLS; PLASMA; SERUM; STRESS; BRAIN; 24S-HYDROXYCHOLESTEROL; SEPARATION; GC-triple quad MS; Oxysterols; Bile acids; Chemical ionization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Apr 2020 11:48 |
| Last Modified: | 23 Apr 2020 11:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17049 |
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