Park, Jung Sun and Khoi, Pham Ngoc and Joo, Young Eun and Lee, Young Han and Lang, Sven A. and Stoeltzing, Oliver and Jung, Young Do (2013) EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells. INTERNATIONAL JOURNAL OF ONCOLOGY, 42 (3). pp. 1120-1126. ISSN 1019-6439, 1791-2423
Full text not available from this repository. (Request a copy)Abstract
Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in epithelial tumor carcinogenesis. In the present study, we examined the effect of epigallocatechin-3-gallate (EGCG), the major green tea catechin, on the induction of RON and tumor growth in human gastric cancer. EGCG inhibited phorbol 12-myristate 13-acetate (PMA)-induced RON expression and reduced RON transcriptional activity. However, (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG) and (-)-epicatechin (EC) did not affect RON expression. Experiments with deleted and site-directed mutagenesis of the RON promoter indicated that Egr-1 binding sites in the RON promoter may be the EGCG-response element acting as a cis-element in gastric cancer cells. EGCG also inhibited PMA-induced Egr-1 expression and DNA binding in a dose-dependent manner. Furthermore, gastric cancer cells pretreated with PMA showed markedly enhanced invasiveness, which was partially abrogated by EGCG and siRNA-targeted RON and Egr-1. EGCG significantly reduced tumor growth in an in vivo tumor model, whereas RON expression was downregulated. These results suggest that EGCG may exert at least part of its anticancer effect by controlling RON expression through suppression of Egr-1 activation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPIDERMAL-GROWTH-FACTOR; MACROPHAGE-STIMULATING PROTEIN; FINGER TRANSCRIPTION FACTOR; HUMAN COLON-CARCINOMA; TYROSINE KINASE; GREEN TEA; (-)-EPIGALLOCATECHIN GALLATE; EPITHELIAL-CELLS; UP-REGULATION; RON; epigallocatechin-3-gallate; recepteur d'origine nantais; Egr-1; gastric cancer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Apr 2020 06:14 |
| Last Modified: | 24 Apr 2020 06:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17105 |
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