Valadares, Napoleao F. and de Oliveira-Silva, Rodrigo and Cavini, Italo A. and Marques, Ivo de Almeida and Pereira, Humberto D'Muniz and Soares-Costa, Andrea and Henrique-Silva, Flavio and Kalbitzer, Hans R. and Munte, Claudia E. and Garratt, Richard C. (2013) X-ray crystallography and NMR studies of domain-swapped canecystatin-1. FEBS JOURNAL, 280 (4). pp. 1028-1038. ISSN 1742-464X, 1742-4658
Full text not available from this repository. (Request a copy)Abstract
The three-dimensional structure of canecystatin-1, a potent inhibitor of cysteine proteases from sugarcane (Saccharumofficinarum), has been solved in two different crystal forms. In both cases, it is seen to exist as a domain-swapped dimer, the first such observation for a cystatin of plant origin. Size exclusion chromatography and multidimensional NMR spectroscopy show the dimer to be the dominant species in solution, despite the presence of a measurable quantity of monomer undergoing slow exchange. The latter is believed to be the active species, whereas the domain-swapped dimer is presumably inactive, as its first inhibitory loop has been extended to form part of a long -strand that forms a double-helical coiled coil with its partner from the other monomer. A similar structure is observed in human cystatinC, but the spatial disposition of the two lobes of the dimer is rather different. Dimerization is presumably a mechanism by which canecystatin-1 can be kept inactive within the plant, avoiding the inhibition of endogenous proteases. The structure described here provides a platform for the rational design of specific cysteine protease inhibitors for biotechnological applications. Database The coordinates and structure factors have been deposited in the Protein Data Bank under the accession codes 3UL5 and 3UL6. Structured digital abstract Canecystatin-1andCanecystatin-1bindbymolecular sieving(View Interaction:1,2) Canecystatin-1andCanecystatin-1bindbynuclear magnetic resonance(View interaction) Canecystatin-1andCanecystatin-1bindbydynamic light scattering(View interaction) Canecystatin-1andCanecystatin-1bindbyx-ray crystallography(View interaction)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN CYSTATIN-C; CRYSTAL-STRUCTURE; CATHEPSIN-B; STRUCTURAL-PROPERTIES; PROTEINASE-INHIBITOR; SECONDARY STRUCTURE; ORYZACYSTATIN-I; PLANT CYSTATINS; BETA-SHEETS; STEFIN-B; domain-swapped; NMR; phytocystatin; X-ray crystallography |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Apr 2020 12:23 |
| Last Modified: | 24 Apr 2020 12:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17194 |
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