Berends, Elsa and Lehle, Ludwig and Henquet, Maurice and Hesselink, Thamara and Woesten, Han A. B. and Lugones, Luis G. and Bosch, Dirk (2013) Identification of alg3 in the mushroom-forming fungus Schizophyllum commune and analysis of the Delta alg3 knockout mutant. GLYCOBIOLOGY, 23 (2). pp. 147-154. ISSN 0959-6658,
Full text not available from this repository. (Request a copy)Abstract
Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene of the mushroom-forming fungus Schizophyllum commune by homology search. Its function was confirmed by the complementation of the Delta alg3 strain of S. cerevisiae. Inactivation of alg3 in S. commune resulted in the production of predominantly Man(3)GlcNAc(2) protein-linked N-glycans. No impact on growth nor a developmental phenotype due to the deletion was observed. This provides a first step toward engineering of a homogeneous, humanized N-glycosylation pattern for the production of therapeutic glycoproteins in mushrooms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; N-GLYCAN; GENE; GLYCOPROTEINS; ENZYME; MANNOSYLTRANSFERASE; GLUCOCEREBROSIDASE; OLIGOSACCHARIDES; GLYCOSYLATION; THERAPY; alg3; basidiomycete; fungus; glycosylation Schizophyllum commune |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Apr 2020 07:08 |
| Last Modified: | 27 Apr 2020 07:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17231 |
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