Neubauer, Bjoern and Machura, Katharina and Kettl, Ramona and Lopez, Maria Luisa S. Sequeira and Friebe, Andreas and Kurtz, Armin (2013) Endothelium-Derived Nitric Oxide Supports Renin Cell Recruitment Through the Nitric Oxide-Sensitive Guanylate Cyclase Pathway. HYPERTENSION, 61 (2). 400-+. ISSN 0194-911X,
Full text not available from this repository. (Request a copy)Abstract
Chronic challenge of renin-angiotensin causes recruitment of renin-producing cells in the kidney along the media layer of afferent arterioles and hypertrophy of cells in the juxtaglomerular apparatus. This study aimed to define the role of nitric oxide (NO) with regard to the recruitment pattern of renin-producing cells and to the possible pathways along which NO could act. We considered the hypothesis that endothelium-derived NO acts via NO-sensitive guanylate cyclase. Mice were treated with low-salt diet in combination with the angiotensin I-converting enzyme inhibitor enalapril for 3 weeks, which led to a 13-fold increase in renin expression associated with marked recruitment of renin cells in afferent arterioles and hypertrophy of the juxtaglomerular apparatus in wild-type mice. In wild-type mice additionally treated with the nonselective NO synthase inhibitor L-NAME, the recruitment of renin-expressing cells along the afferent arterioles was absent and juxtaglomerular hypertrophy was diminished. An almost identical attenuation of renin cell recruitment as with L-NAME treatment in wild-type mice was found in mice lacking the endothelial isoform of NO synthase. Treatment of mice lacking NO-sensitive guanylate cyclase in renin-expressing cells and preglomerular smooth muscle cells with low-salt diet in combination with the angiotensin I-converting enzyme inhibitor enalapril for 3 weeks produced juxtaglomerular hypertrophy like in wild-type mice, but no recruitment in afferent arterioles. These findings suggest that endothelium-derived NO and concomitant formation of cGMP in preglomerular renin cell precursors supports recruitment of renin-expressing cells along preglomerular vessels, but not in the juxtaglomerular apparatus. (Hypertension. 2013;61:400-407.) circle Online Data Supplement
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | JUXTAGLOMERULAR CELLS; BLOOD-PRESSURE; RAT-KIDNEY; GENE-EXPRESSION; KNOCKOUT MICE; INDUCED HYPERTENSION; ANGIOTENSIN SYSTEM; PERFUSION-PRESSURE; MESSENGER-RNA; SECRETION; guanylate cyclase; juxtaglomerular apparatus; nitric oxide; recruitment; renin |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Apr 2020 07:36 |
| Last Modified: | 27 Apr 2020 07:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17238 |
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