Herzner, Gudrun and Schlecht, Anja and Dollhofer, Veronika and Parzefall, Christopher and Harrar, Klaus and Kreuzer, Andreas and Pilsl, Ludwig and Ruther, Joachim (2013) Larvae of the parasitoid wasp Ampulex compressa sanitize their host, the American cockroach, with a blend of antimicrobials. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110 (4). pp. 1369-1374. ISSN 0027-8424,
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Food resources contaminated with spoilage or pathogenic microorganisms pose severe problems to all higher organisms. Here, we describe a food-hygienic strategy of the emerald cockroach wasp Ampulex compressa. The wasp larvae develop on and inside the American cockroach Periplaneta americana, a host that can harbor various putrefactive microbes, as well as human and insect pathogens. From P. americana, we isolated the Gram-negative bacterium Serratia marcescens, which is a potent entomopathogen that can rapidly kill insect larvae. It is also known as a food contaminant and as an opportunistic human pathogen. Using behavioral observations and chemical analyses, we demonstrated that A. compressa larvae impregnate their cockroach hosts from inside with large amounts of an oral secretion containing a blend of gamma-lactones and isocoumarins with (R)-(-)-mellein [(R)-(-)-3,4-diydro-8-hydroxy-3-methylisocoumarin] and micromolide [(4R,9Z)-octadec-9-en-4-olide] as dominant components. We fractionated hexane extracts of the secretion and investigated the antimicrobial properties of the fraction containing the lactones and isocoumarins, as well as of synthetic (R)-(-)-mellein and micromolide, against S. marcescens and a Gram-positive bacterium, Staphylococcus hyicus, in broth microdilution assays. The test fraction inhibited growth of both tested bacteria. The activity of the fraction against S. marcescens was explained by (R)-(-)-mellein alone, and the activity against S. hyicus was explained by the combined action of (R)-(-)-mellein and micromolide. Our data suggest that the specific combination of antimicrobials in the larval secretion provides an effective frontline defense against the unpredictable spectrum of microbes that A. compressa larvae may encounter during their development inside their cockroach hosts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CURRANT STEM GIRDLER; SERRATIA-MARCESCENS; PERIPLANETA-AMERICANA; NOSOCOMIAL INFECTIONS; ASPERGILLUS-OCHRACEUS; PATHOGENIC BACTERIA; BLATTELLA-GERMANICA; MEDICAL IMPORTANCE; POTENTIAL VECTORS; SEX-PHEROMONE; antibiotic; food hygiene; jewel wasp |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Evolutionary Ecology (Prof. Dr. Erhard Strohm) Biology, Preclinical Medicine > Institut für Zoologie > Chemische Ökologie (Prof. Dr. Joachim Ruther) Chemistry and Pharmacy > Institut für Organische Chemie Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Oliver Reiser |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Apr 2020 06:09 |
| Last Modified: | 28 Apr 2020 06:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17277 |
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