Karl, Matthias and Spoerner, Michael and Thuy-Vy Pham, and Narayanan, Sunilkumar Puthenpurackal and Kremer, Werner and Kalbitzer, Hans Robert (2017) Pressure response of P-31 chemical shifts of adenine nucleotides. BIOPHYSICAL CHEMISTRY, 231. pp. 50-54. ISSN 0301-4622, 1873-4200
Full text not available from this repository. (Request a copy)Abstract
High pressure NMR spectroscopy is a powerful method for identifying rare conformational states of proteins from the pressure response of their chemical shifts. Many proteins have bound adenine nucleotides at their active centers, in most cases in a complex with Mg2+-ions. The P-31 NMR signals of phosphate groups of the nucleotides can be used as probes for conformational transitions in the proteins themselves. For distinguishing protein specific pressure effects from trivial pressure responses not due to the protein interaction, data of the pressure response of the free nucleotides must be available. Therefore, the pressure response of P-31 chemical shifts of the adenine nucleotides AMP, ADP, and ATP and their Mg2+-complexes has been determined at pH values several units distant from the respective pk-values. It is clearly non-linear for most of the resonances. A negative first order pressure coefficient B-1 was determined for all P-31 resonances except Mg2+center dot AMP indicating an upfield shift of the resonances with pressure. The smallest and largest negative values are obtained for the alpha-phosphate group of ADP and beta-phosphate group of Mg2+center dot ATP with -0.32 and -4.59 ppm/GPa, respectively. With exception of the alpha-phosphate group of Mg2+center dot AMP the second order pressure coefficients are positive leading to a saturation like behaviour. The pressure response of the adenine nucleotides is similar but not identical to that observed earlier for guanine nucleotides. The obtained data show that the chemical shift pressure response of the different phosphate groups is rather different dependent on the position of phosphate group in the nucleotide and the nucleotide used. (C) 2017 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; ADENYLATE KINASE; METAL-ION; NMR; ATP; PHOSPHORUS; PROTEINS; COMPLEX; P-31 NMR; High pressure NMR spectroscopy; Chemical shift; Adenine nucleotides |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:18 |
| Last Modified: | 13 Dec 2019 13:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1731 |
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