Gerlach, Roman G. and Walter, Steffi and McClelland, Michael and Schmidt, Christiane and Steglich, Matthias and Prager, Rita and Bender, Jennifer K. and Fuchs, Stephan and Schoerner, Christoph and Rabsch, Wolfgang and Lang, Werner and Jantsch, Jonathan (2017) Comparative whole genome analysis of three consecutive Salmonella diarizonae isolates. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 307 (8). pp. 542-551. ISSN 1438-4221, 1618-0607
Full text not available from this repository. (Request a copy)Abstract
Infections of very young children or immunocompromised people with Salmonella of higher subspecies are a well-known phenomenon often associated with contact to cold-blooded animals. We describe the molecular characterization of three S. enterica subsp. diarizonae strains, isolated consecutively over a period of several months from a hospital patient suffering from diarrhea and sepsis with fatal outcome. With the initial isolate the first complete genome sequence of a member of subsp. diarizonae is provided and based on this reference we revealed the genomic differences between the three isolates by use of next-generation sequencing and confirmed by phenotypical tests. Genome comparisons revealed mutations within gpt, hfq and purK in the first isolate as a sign of clonal variation rather than host-directed evolution. Furthermore, our work demonstrates that S. enterica subsp. diarizonae possess, besides a conserved set of known Salmonella Pathogenicity Islands, a variable portfolio of additional genomic islands of unknown function.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MULTIPLE ALIGNMENT; SEQUENCE ALIGNMENT; ESCHERICHIA-COLI; TYPHIMURIUM; ENTERICA; PERFORMANCE; INFECTION; VIRULENCE; IIIB; HFQ; Salmonella; Sepsis; Diarizonae; subsp IIIb; Comparative genomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 13 Feb 2019 14:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1732 |
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