Rogler, Anja and Hoja, Sabine and Socher, Eileen and Nolte, Elke and Wach, Sven and Wieland, Wolf and Hofstaedter, Ferdinand and Goebell, Peter J. and Wullich, Bernd and Hartmann, Arndt and Stoehr, Robert (2013) Role of two single nucleotide polymorphisms in secreted frizzled related protein 1 and bladder cancer risk. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 6 (10). pp. 1984-1998. ISSN 1936-2625,
Full text not available from this repository. (Request a copy)Abstract
In this study, we determined the genotype distribution of two single nucleotide polymorphisms (SNPs) in secreted frizzled related protein 1 (SFRP1), rs3242 and rs921142, in a Caucasian bladder cancer case-control study. Allelic variants of the SNPs were determined using restriction fragment length polymorphism (RFLP) analysis and partly verified by sequencing analysis. Overall, DNA from 188 consecutive and 215 early-onset bladder cancer patients (<= 45 years) as well as from 332 controls was investigated. Potential microRNA binding sites were determined for rs3242, and microRNA expression was analysed in cell lines and tumour specimens. We observed a remarkable distribution difference in rs3242 between bladder cancer patients and healthy controls (p=0.05). Additionally, we found a significant difference in genotype distribution (p=0.032), resulting from the difference of early-onset patients and the control group (p=0.007). The risk allele T showed increased frequency in the early-onset patient group (p=0.002). Genotype-dependent differences of microRNA binding capacity were predicted in SFRP1 mRNA for two microRNAs. Hsa-miR-3646 showed strong expression in cell lines and tumour tissue, whereas hsa-miR-603 exhibited weak expression. The rs921142 SNP showed no significant association with bladder cancer risk. This is the first study to describe an association of the SFRP1 SNP rs3242 and bladder cancer risk as well as the influence of rs3242 on genotype-dependent microRNA capacity on SFRP1 mRNA. The onset of bladder seems to be associated with the increased occurrence of the T-allele in rs3242.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSITIONAL-CELL-CARCINOMA; ACCELERATES TUMOR-FORMATION; WNT ANTAGONIST SFRP1; LI-FRAUMENI-SYNDROME; MDM2 SNP309; P53 MUTATION; PATHWAY; ASSOCIATION; LINES; METHYLATION; SFRP1; SNP; bladder cancer; microRNA; Wnt signalling pathway |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie Medicine > Lehrstuhl für Urologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Apr 2020 09:46 |
| Last Modified: | 28 Apr 2020 09:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17333 |
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