Gitlin, Leonid and Hoera, Christian and Meier, Robert J. and Nagl, Stefan and Belder, Detlev (2013) Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring. LAB ON A CHIP, 13 (20). pp. 4134-4141. ISSN 1473-0197,
Full text not available from this repository. (Request a copy)Abstract
Real-time chemical reaction monitoring in microfluidic environments is demonstrated using luminescent chemical sensors integrated in PDMS/glass-based microscale reactors. A fabrication procedure is presented that allows for straightforward integration of thin polymer layers with optical sensing functionality in microchannels of glass-PDMS chips of only 150 mm width and of 10 to 35 mm height. Sensor layers consisting of polystyrene and an oxygen-sensitive platinum porphyrin probe with film thicknesses of about 0.5 to 4 mm were generated by combining spin coating and abrasion techniques. Optimal coating procedures were developed and evaluated. The chip-integrated sensor layers were calibrated and investigated with respect to stability, reproducibility and response times. These microchips allowed observation of dissolved oxygen concentration in the range of 0 to over 40 mg L-1 with a detection limit of 368 mu g L-1. The sensor layers were then used for observation of a model reaction, the oxidation of sulphite to sulphate in a microfluidic chemical reactor and could observe sulphite concentrations of less than 200 mu M. Real-time on-line monitoring of this chemical reaction was realized at a fluorescence microscope setup with 405 nm LED excitation and CCD camera detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOTAL ANALYSIS SYSTEMS; ANALYTICAL SEPARATION; MICROFLUIDIC DEVICE; LATEST DEVELOPMENTS; OXYGEN SENSORS; CELL BIOLOGY; ELECTROPHORESIS; CHROMATOGRAPHY; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 05:10 |
| Last Modified: | 29 Apr 2020 05:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17369 |
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