Gorris, Hans H. and Wolfbeis, Otto S. (2013) Photon-Upconverting Nanoparticles for Optical Encoding and Multiplexing of Cells, Biomolecules, and Microspheres. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52 (13). pp. 3584-3600. ISSN 1433-7851, 1521-3773
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Photon-upconverting nanoparticles (UCNPs) are lanthanide-doped nanocrystals that emit visible light under near-infrared excitation (anti-Stokes emission). This unique optical property precludes background fluorescence and light scattering from biological materials. The emission of multiple and narrow emission lines is an additional hallmark of UCNPs that opens up new avenues for optical encoding. Distinct emission signatures can be obtained if the multiple emission of UCNPs is tuned by their dopant composition or by surface modification with dyes. Tuning the intensity of only one of the multiple emission lines and using another one as a constant reference signal enables the design of ratiometric codes that are resistant to fluctuations in absolute signal intensities. Combining several UCNPs each displaying a distinct set of emission lines expands the coding capacity exponentially and lays the foundation for highly multiplexed analyte detection. This Review highlights the potential of UCNPs for labeling and encoding biomolecules, microspheres, and even whole cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UP-CONVERSION FLUORESCENCE; RESONANCE ENERGY-TRANSFER; CONVERTING PHOSPHOR REPORTERS; NEAR-INFRARED LIGHT; IN-VIVO; QUANTUM DOTS; PHOTODYNAMIC THERAPY; LATERAL-FLOW; HOMOGENEOUS IMMUNOASSAY; SILICA NANOPARTICLES; anti-Stokes emission; lanthanides; nanoparticles; optical encoding; photon upconversion |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Petra Gürster |
| Date Deposited: | 27 May 2020 08:04 |
| Last Modified: | 27 May 2020 08:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17445 |
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