Wilhelm, Stefan and Hirsch, Thomas and Patterson, Wendy M. and Scheucher, Elisabeth and Mayr, Torsten and Wolfbeis, Otto S. (2013) Multicolor Upconversion Nanoparticles for Protein Conjugation. THERANOSTICS, 3 (4). pp. 239-248. ISSN 1838-7640,
Full text not available from this repository. (Request a copy)Abstract
We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e. g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAGNETIC NANOPARTICLES; QUANTUM DOTS; UPCONVERTING NANOPARTICLES; IN-VIVO; NANOCRYSTALS; PHASE; BIOCONJUGATION; NANOPHOSPHORS; LUMINESCENCE; Upconversion luminescence; nanoparticle; silica coating; Raman spectroscopy; bio-sensing; magnetic separation; surface plasmon resonance |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 06:40 |
| Last Modified: | 29 Apr 2020 06:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17449 |
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