Hierlmeier, Thomas and Merl, Juliane and Sauert, Martina and Perez-Fernandez, Jorge and Schultz, Patrick and Bruckmann, Astrid and Hamperl, Stephan and Ohmayer, Uli and Rachel, Reinhard and Jacob, Anja and Hergert, Kristin and Deutzmann, Rainer and Griesenbeck, Joachim and Hurt, Ed and Milkereit, Philipp and Bassler, Jochen and Tschochner, Herbert (2013) Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S-cerevisiae. NUCLEIC ACIDS RESEARCH, 41 (2). pp. 1191-1210. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
Eukaryotic ribosome biogenesis requires more than 150 auxiliary proteins, which transiently interact with pre-ribosomal particles. Previous studies suggest that several of these biogenesis factors function together as modules. Using a heterologous expression system, we show that the large ribosomal subunit (LSU) biogenesis factor Noc1p of Saccharomyces cerevisiae can simultaneously interact with the LSU biogenesis factor Noc2p and Rrp5p, a factor required for biogenesis of the large and the small ribosomal subunit. Proteome analysis of RNA polymerase-I-associated chromatin and chromatin immunopurification experiments indicated that all members of this protein module and a specific set of LSU biogenesis factors are co-transcriptionally recruited to nascent ribosomal RNA (rRNA) precursors in yeast cells. Further ex vivo analyses showed that all module members predominantly interact with early pre-LSU particles after the initial pre-rRNA processing events have occurred. In yeast strains depleted of Noc1p, Noc2p or Rrp5p, levels of the major LSU pre-rRNAs decreased and the respective other module members were associated with accumulating aberrant rRNA fragments. Therefore, we conclude that the module exhibits several binding interfaces with pre-ribosomes. Taken together, our results suggest a co- and post-transcriptional role of the yeast Rrp5p-Noc1p-Noc2p module in the structural organization of early LSU precursors protecting them from non-productive RNase activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMERASE-I TRANSCRIPTION; FUNCTIONAL-CHARACTERIZATION; NUCLEAR EXPORT; ESSENTIAL GENE; YEAST HOMOLOG; SITE A(2); U3 SNORNP; RNA GENES; BIOGENESIS; COMPLEX; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 08:31 |
| Last Modified: | 29 Apr 2020 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17491 |
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