Armache, Jean-Paul and Anger, Andreas M. and Marquez, Viter and Franckenberg, Sibylle and Froehlich, Thomas and Villa, Elizabeth and Berninghausen, Otto and Thomm, Michael and Arnold, Georg J. and Beckmann, Roland and Wilson, Daniel N. (2013) Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution. NUCLEIC ACIDS RESEARCH, 41 (2). pp. 1284-1293. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. Molecular models have been reported for complete bacterial 70S and eukaryotic 80S ribosomes; however, only molecular models of large 50S subunits have been reported for archaea. Here, we present a complete molecular model for the Pyrococcus furiosus 70S ribosome based on a 6.6 A cryo-electron microscopy map. Moreover, we have determined cryo-electron microscopy reconstructions of the Euryarchaeota Methanococcus igneus and Thermococcus kodakaraensis 70S ribosomes and Crenarchaeota Staphylothermus marinus 50S subunit. Examination of these structures reveals a surprising promiscuous behavior of archaeal ribosomal proteins: We observe intersubunit promiscuity of S24e and L8e (L7ae), the latter binding to the head of the small subunit, analogous to S12e in eukaryotes. Moreover, L8e and L14e exhibit intrasubunit promiscuity, being present in two copies per archaeal 50S subunit, with the additional binding site of L14e analogous to the related eukaryotic r-protein L27e. Collectively, these findings suggest insights into the evolution of eukaryotic ribosomal proteins through increased copy number and binding site promiscuity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CRYO-EM STRUCTURE; CRYSTAL-STRUCTURE; ELECTRON-MICROSCOPY; BACTERIAL RIBOSOME; STRUCTURAL BASIS; SP-NOV; RNA; SUBUNIT; COMPLEX; VISUALIZATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 08:34 |
| Last Modified: | 29 Apr 2020 08:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17492 |
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