Mueller, Michael B. and Blunk, Torsten and Appel, Bernhard and Maschke, Angelika and Goepferich, Achim and Zellner, Johannes and Englert, Carsten and Prantl, Lukas and Kujat, Richard and Nerlich, Michael and Angele, Peter (2013) Insulin is essential for in vitro chondrogenesis of mesenchymal progenitor cells and influences chondrogenesis in a dose-dependent manner. INTERNATIONAL ORTHOPAEDICS, 37 (1). pp. 153-158. ISSN 0341-2695,
Full text not available from this repository. (Request a copy)Abstract
Insulin is a commonly used additive in chondrogenic media for differentiating mesenchymal stem cells (MSCs). The indispensability of other bioactive factors like TGF-beta or dexamethasone in these medium formulations has been shown, but the role of insulin is unclear. The purpose of this study was to investigate whether insulin is essential for MSC chondrogenesis and if there is a dose-dependent effect of insulin on MSC chondrogenesis. We cultivated human MSCs in pellet culture in serum-free chondrogenic medium with insulin concentrations between 0 and 50 mu g/ml and assessed the grade of chondrogenic differentiation by histological evaluation and determination of glycosaminoglycan (GAG), total collagen and DNA content. We further tested whether insulin can be delivered in an amount sufficient for MSC chondrogenesis via a drug delivery system in insulin-free medium. Chondrogenesis was not induced by standard chondrogenic medium without insulin and the expression of cartilage differentiation markers was dose-dependent at insulin concentrations between 0 and 10 mu g/ml. An insulin concentration of 50 mu g/ml had no additional effect compared with 10 mu g/ml. Insulin was delivered by a release system into the cell culture under insulin-free conditions in an amount sufficient to induce chondrogenesis. Insulin is essential for MSC chondrogenesis in this system and chondrogenic differentiation is influenced by insulin in a dose-dependent manner. Insulin can be provided in a sufficient amount by a drug delivery system. Therefore, insulin is a suitable and inexpensive indicator substance for testing drug release systems in vitro.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GROWTH-FACTOR-I; STEM-CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; OSTEOGENIC PROTEIN-1; CULTURE-MEDIUM; CARTILAGE; DIFFERENTIATION; PROLIFERATION; TISSUE; CHONDROCYTES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 10:43 |
| Last Modified: | 29 Apr 2020 10:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17524 |
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