Tosoni, Kendra and Stobbart, Michelle and Cassidy, Diane M. and Venerando, Andrea and Pagano, Mario A. and Luz, Simao and Amaral, Margarida D. and Kunzelmann, Karl and Pinna, Lorenzo A. and Farinha, Carlos M. and Mehta, Anil (2013) CFTR mutations altering CFTR fragmentation. BIOCHEMICAL JOURNAL, 449. pp. 295-305. ISSN 0264-6021, 1470-8728
Full text not available from this repository. (Request a copy)Abstract
Most CF (cystic fibrosis) results from deletion of a phenylalanine (F-508) in the CFTR {CF transmembrane-conductance regulator; ABCC7 [ABC (ATP-binding cassette) sub-family C member 7]} which causes ER (endoplasmic reticulum) degradation of the mutant. Using stably CFTR-expressing BHK (baby-hamster kidney) cell lines we demonstrated that wild-type CTIR and the F508delCFTR mutant are cleaved into differently sized N- and C-terminal-bearing fragments, with each hemi-CFTR carrying its nearest NBD (nucleotide-binding domain), reflecting differential cleavage through the central CFTR R-domain. Similar NBD1-bearing fragments are present in the natively expressing HBE (human bronchial epithelial) cell line. We also observe multiple smaller fragments of different sizes in BHK cells, particularly after F508del mutation (ladder pattern). Trapping wild-type CFT'R in the ER did not generate a F508del fragmentation fingerprint. Fragments change their size/pattern again post-mutation at sites involved in CFTR's in vitro interaction with the pleiotropic protein kinase CK2 (S511A in NBD1). The F508del and S511A mutations generate different fragmentation fingerprints that are each unlike the wild-type; yet, both mutants generate new N-terminal-bearing CFTR fragments that are not observed with other CK2-related mutations (S511D, S422A/D and T1471A/D). We conclude that the F508delCFTR mutant is not degraded completely and there exists a relationship between CFTR's fragmentation fingerprint and the CFTR sequence through putative CK2-interactive sites that lie near F508.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-KINASE CK2; TRANSMEMBRANE CONDUCTANCE REGULATOR; CYSTIC-FIBROSIS; CALPAIN; DELTA-F508-CFTR; IDENTIFICATION; DEGRADATION; CLEARANCE; CHANNEL; F508; casein kinase 2 (CK2); cystic fibrosis; cystic fibrosis transmembrane-conductance regulator (CFTR); fragmentation; Delta F508-CFTR mutation; F508delCFTR |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Apr 2020 11:42 |
| Last Modified: | 29 Apr 2020 11:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17539 |
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