Liebherr, Raphaela B. and Soukka, Tero and Wolfbeis, Otto S. and Gorris, Hans H. (2012) Maleimide activation of photon upconverting nanoparticles for bioconjugation. NANOTECHNOLOGY, 23 (48): 485103. ISSN 0957-4484,
Full text not available from this repository. (Request a copy)Abstract
Photon upconverting nanoparticles (UCNPs) have become an important new class of optical labels. Their unique property of emitting visible light after photo-excitation with near-infrared radiation enables biological imaging without background interference or cell damage. Biological applications require UCNPs that are dispersible in water and allow the attachment of biomolecules. Oleic acid-coated UCNPs obtained by solvothermal synthesis were functionalized with both hydrophilic PEG and thiol-reactive maleimides, either by ligand exchange or by silanization. Three different types of maleimide-functionalized UCNPs were prepared and characterized by transmission electron microscopy, dynamic light scattering and Raman spectroscopy. Ligand exchange of oleic acid by maleimide-PEG-COOH yielded UCNPs that did not aggregate, were colloidally stable and reacted readily with proteins. Such luminescent labels are required for background-free imaging and many other bioanalytical applications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | UP-CONVERSION FLUORESCENCE; LUMINESCENT NANOPARTICLES; RAMAN-SPECTRA; FUNCTIONALIZATION; NANOCRYSTALS; CHEMISTRY; PHOSPHORS; STRATEGY; LABELS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Apr 2020 12:10 |
| Last Modified: | 30 Apr 2020 12:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/17607 |
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