Matysik, Silke and Liebisch, Gerhard (2017) Quantification of steroid hormones in human serum by liquid chromatography-high resolution tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1526. pp. 112-118. ISSN 0021-9673, 1873-3778
Full text not available from this repository. (Request a copy)Abstract
A limited specificity is inherent to immunoassays for steroid hormone analysis. To improve selectivity mass spectrometric analysis of steroid hormones by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been introduced in the clinical laboratory over the past years usually with low mass resolution triple-quadrupole instruments or more recently by high resolution mass spectrometry (HR-MS). Here we introduce liquid chromatography-high resolution tandem mass spectrometry (LC-MS/HR-MS) to further increase selectivity of steroid hormone quantification. Application of HR-MS demonstrates an enhanced selectivity compared to low mass resolution. Separation of isobaric interferences reduces background noise and avoids overestimation. Samples were prepared by automated liquid-liquid extraction with MTBE. The LC-MS/HR-MS method using a quadrupole-Orbitrap analyzer includes eight steroid hormones i.e. androstenedione, corticosterone, cortisol, cortisone, 11-deoxycortisol, 17-hydroxyprogesterone, progesterone, and testosterone. It has a run-time of 5.3 min and was validated according to the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) guidelines, For most of the analytes coefficient of variation were 10% or lower and LOQs were determined significantly below 1 ng/ml. Full product ion spectra including accurate masses substantiate compound identification by matching their masses and ratios with authentic standards. In summary, quantification of steroid hormones by LC-MS/HR-MS is applicable for clinical diagnostics and holds also promise for highly selective quantification of other small molecules. (C) 2017 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LC-MS/MS; ANABOLIC-STEROIDS; VALIDATION; EXTRACTION; PLASMA; CORTISOL; ACCURATE; BLOOD; High resolution mass spectrometry; Liquid chromatography; Steroid hormones; Quantification; Increase of selectivity; Orbitrap |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 13 Dec 2019 14:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1792 |
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