Granafei, Sara and Liebisch, Gerhard and Palmisano, Francesco and Carlucci, Roberto and Lionetti, Adriana and Longobardi, Francesco and Bianco, Giuliana and Cataldi, Tommaso R. I. (2017) Effect of Storage and Extraction Protocols on the Lipid and Fatty Acid Profiles of Dicentrarchus labrax Brain. FOOD ANALYTICAL METHODS, 10 (12). pp. 4003-4012. ISSN 1936-9751, 1936-976X
Full text not available from this repository. (Request a copy)Abstract
Fish brain might represent an interesting raw material since it is particularly enriched in lipids (e.g., phosphatidylcholines (PCs)) that are valuable food additives and nutraceuticals. A chloroform-free solvent extraction protocol based on 2-methoxy-2-methylpropane also known as methyl tert-butyl ether (MTBE) was evaluated and compared with CHCl3:MeOH mixtures of the classical Bligh-Dyer method. Phospholipids (PLs) and sphingolipids (SPs) occurring in the brain extracts of farmed European sea bass fish (Dicentrarchus labrax) were identified/analyzed by flow injection-electrospray ionization-mass spectrometry (FI-ESI-MS), whereas the content and identity of fatty acids (FA) were obtained by gas chromatography-MS upon derivatization as FA methyl esters. On the basis of these preliminary results, the total contents of PL and SP, as well as their relative abundances, were not significantly affected by the extraction protocol used. The most abundant lipid class was the PC with a mean +/- SD content (expressed as nmol/mg protein) of 507 +/- 89, followed by phosphoethanolamines (195 +/- 41), ceramides (35 +/- 9), sphingomyelins (9 +/- 4), lyso-phosphatidylcholines (8 +/- 2), and phosphatidylglycerols (1.8 +/- 0.8). In terms of pre-processing strategy of fish brain samples, two preservation methods, i.e., freezing and freeze-drying, were also compared. Lyophilization led to a partial degradation of PC to their corresponding lyso-form, most likely as a result of endogenous phospholipase activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; INTERACTION LIQUID-CHROMATOGRAPHY; HIGH-THROUGHPUT QUANTIFICATION; BREAM SPARUS-AURATA; THERMALLY STRESSED MUSSELS; ELECTROSPRAY-IONIZATION; CARDIOPROTECTIVE ACTIVITIES; OLIVE POMACE; POLAR LIPIDS; BY-PRODUCTS; Fish brain; Food waste; Lipids; Mass spectrometry; Methyl tert-butyl ether; Dicentrarchus labrax; Freeze-drying |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 19 Feb 2019 07:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1807 |
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