Riquelme, Paloma and Amodio, Giada and Macedo, Camila and Moreau, Aurelie and Obermajer, Natasa and Brochhausen, Christoph and Ahrens, Norbert and Kekarainen, Tuija and Faendrich, Fred and Cuturi, Cristina and Gregori, Silvia and Metes, Diana and Schlitt, Hans J. and Thomson, Angus W. and Geissler, Edward K. and Hutchinson, James A. (2017) DHRS9 Is a Stable Marker of Human Regulatory Macrophages. TRANSPLANTATION, 101 (11). pp. 2731-2738. ISSN 0041-1337, 1534-6080
Full text not available from this repository. (Request a copy)Abstract
Background. The human regulatory macrophage (Mreg) has emerged as a promising cell type for use as a cell-based adjunct immunosuppressive therapy in solid organ transplant recipients. In this brief report, dehydrogenase/reductase 9 (DHRS9) is identified as a robust marker of human Mregs. Methods. The cognate antigen of a mouse monoclonal antibody raised against human Mregs was identified as DHRS9 by immunoprecipitation and MALDI-MS sequencing. Expression of DHRS9 within a panel of monocyte-derived macrophages was investigated by quantitative PCR, immunoblotting and flow cytometry. Results. DHRS9 expression discriminated human Mregs from a panel of in vitro derived macrophages in other polarisation states. Likewise, DHRS9 expression distinguished Mregs from a variety of human monocyte-derived tolerogenic antigen-presenting cells in current development as cell-based immunotherapies, including Tol-DC, Rapa-DC, DC-10, and PGE2-induced myeloid-derived suppressor cells. A subpopulation of DHRS9-expressing human splenic macrophages was identified by immunohistochemistry. Expression of DHRS9 was acquired gradually during in vitro development of human Mregs from CD14(+) monocytes and was further enhanced by IFN-gamma treatment on day 6 of culture. Stimulating Mregs with 100 ng/mL lipopolysaccharide for 24 hours did not extinguish DHRS9 expression. Dhrs9 was not an informative marker of mouse Mregs. Conclusion. DHRS9 is a specific and stable marker of human Mregs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN DENDRITIC CELLS; RETINOIC ACID; SUPPRESSOR-CELLS; MYELOID CELLS; T-CELL; EXPRESSION; DIFFERENTIATION; TRANSPLANTATION; METABOLISM; ACTIVATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 01 Mar 2019 08:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1885 |
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