Moriniere, Jerome and Hendrich, Lars and Balke, Michael and Beermann, Arne J. and Koenig, Tobias and Hess, Monika and Koch, Stefan and Mueller, Reinhard and Leese, Florian and Hebert, Paul D. N. and Hausmann, Axel and Schubart, Christoph D. and Haszprunar, Gerhard (2017) A DNA barcode library for Germanys mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera). MOLECULAR ECOLOGY RESOURCES, 17 (6). pp. 1293-1307. ISSN 1755-098X, 1755-0998
Full text not available from this repository. (Request a copy)Abstract
Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immature life stages, is a challenging, yet fundamental task. A comprehensive DNA barcode library based upon taxonomically well-curated specimens is needed to overcome the problematic identification. Once available, this library will support the implementation of fast, cost-efficient and reliable DNA-based identifications and assessments of ecological status. This study represents a major step towards a DNA barcode reference library as it covers for two-thirds of Germany's EPT species including 2,613 individuals belonging to 363 identified species. As such, it provides coverage for 38 of 44 families (86%) and practically all major bioindicator species. DNA barcode compliant sequences (500bp) were recovered from 98.74% of the analysed specimens. Whereas most species (325, i.e., 89.53%) were unambiguously assigned to a single Barcode Index Number (BIN) by its COI sequence, 38 species (18 Ephemeroptera, nine Plecoptera and 11 Trichoptera) were assigned to a total of 89 BINs. Most of these additional BINs formed nearest neighbour clusters, reflecting the discrimination of geographical subclades of a currently recognized species. BIN sharing was uncommon, involving only two species pairs of Ephemeroptera. Interestingly, both maximum pairwise and nearest neighbour distances were substantially higher for Ephemeroptera compared to Plecoptera and Trichoptera, possibly indicating older speciation events, stronger positive selection or faster rate of molecular evolution.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BAETIS-RHODANI EPHEMEROPTERA; VERNUS GROUP EPHEMEROPTERA; SPECIES DELIMITATION; INTEGRATIVE TAXONOMY; CRYPTIC DIVERSITY; BIOLOGICAL IDENTIFICATIONS; MITOCHONDRIAL PSEUDOGENES; BIODIVERSITY; QUALITY; FAUNA; barcode library; bioindicators; COI; cryptic diversity; DNA barcoding; Ephemeroptera; Germany; mitochondrial DNA; Plecoptera; Trichoptera; water quality |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Zoologie/Evolutionsbiologie (Prof. Dr. Jürgen Heinze) |
| Depositing User: | Petra Gürster |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 04 Sep 2020 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1903 |
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