Hahnel, Sebastian and Muehlbauer, Gudrun and Hoffmann, Judith and Ionescu, Andrei and Buergers, Ralf and Rosentritt, Martin and Handel, Gerhard and Haeberlein, Ingo (2012) Streptococcus mutans and Streptococcus sobrinus biofilm formation and metabolic activity on dental materials. ACTA ODONTOLOGICA SCANDINAVICA, 70 (2). pp. 114-121. ISSN 0001-6357,
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Objectives. To examine potential correlations between streptococcal biofilm formation and lactate production in streptococcal biofilms formed on the surface of dental materials with different surface characteristics. Materials and methods. Samples of a glass-ionomer cement (Ketac Molar) and a ceramic (Empress 2) were incubated with whole saliva and suspensions of Streptococcus mutans ATCC 25175 or Streptococcus sobrinus ATCC 33478 for initiating single-species biofilm formation for either 4 or 24 h. The relative amount of adherent, viable cells was determined using a Resazurin and a MTT assay. Metabolic activity was assessed by quantifying lactate production with a modification of the commercial Clinpro Cario L-Pop kit. Results. Both assays identified similar S. sobrinus biofilm formation on the two substrata; for S. mutans, the MTT test showed significantly fewer streptococci on the glass-ionomer cement than on the ceramic. Concerning metabolic activity, for S. sobrinus, significantly higher lactate production was observed for biofilms formed on the glass-ionomer cement in comparison to the ceramic, whereas similar values were identified for S. mutans. Conclusions. Within the limitations of the study, the results suggest that the pure amount of adherent streptococci does not a priori indicate the metabolic activity of the cariogenic bacteria organized in the respective biofilm. Thus, comparisons between the relative amount of adherent streptococci and their metabolic activity may allow for an improved understanding of the effect of dental material surfaces on the formation and metabolic activity of streptococcal biofilms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESTORATIVE MATERIALS; GLASS-IONOMER; IN-VITRO; BACTERIAL-COLONIZATION; PELLICLE FORMATION; FLUORIDE RELEASE; ACID PRODUCTION; ADHESION; ADHERENCE; COMPOSITE; lactate production; Streptococcus; biofilm; bacterial adhesion |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnärztliche Prothetik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 May 2020 05:59 |
| Last Modified: | 19 May 2020 05:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/19192 |
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