Seyfried, T. F. and Gruber, M. and Pawlik, M. T. and Kasper, S. and Mandle, R. J. and Hansen, E. (2017) A new approach for fat removal in a discontinuous autotransfusion device-concept and evaluation. VOX SANGUINIS, 112 (8). pp. 759-766. ISSN 0042-9007, 1423-0410
Full text not available from this repository. (Request a copy)Abstract
BackgroundFat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of central fat accumulation at the bottom of Latham bowls, a fat reduction program was developed using two volume displacements, where blood temporarily is removed and respun in the bowl to force the fat through the RBC sediment. Materials and methodsPooled ABO-matched RBC and FFP were adjusted to a haematocrit of 10%, and human fat tissue added to a concentration of 1<bold></bold>25 vol%. In six experiments, blood was processed with the new-generation cell salvage device CS Elite in a newly developed fat reduction program in bowls of three sizes. Volumetric quantification of fat was performed after centrifugation of blood samples in Pasteur pipettes. From volumes, haematocrits and the concentrations of fat, RBC recovery and fat elimination rates were calculated. ResultsFat removal rates of 93<bold></bold>22<bold></bold>8, 97<bold></bold>0 +/- 2<bold></bold>1 and 99<bold></bold>6 +/- 0<bold></bold>3% were observed with a 70-ml, 125-ml and 225-ml bowl, respectively, and even higher rates when removal rates were calculated one cycle. At the same time, high RBC recovery and plasma elimination rates were maintained, not significantly different to the default program mode. ConclusionModifications in process parameters and sequence led to a fat reduction program that significantly improves fat removal with the Cell Saver Elite from 77<bold></bold>4 +/- 5<bold></bold>1% in the default mode to an average of 98<bold></bold>6 +/- 1<bold></bold>1%, yielding results equivalent to the continuous cell salvage system (C.A.T.S).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERICARDIAL SUCTION BLOOD; SPONTANEOUS DENSITY SEPARATION; CARDIOPULMONARY BYPASS; SALVAGE DEVICES; CELL SALVAGE; SHED BLOOD; LIQUID FAT; MICROPARTICLES; TRANSFUSION; ELIMINATION; RBC transfusion; transfusion complications- non-infectious; transfusion practices (surgical) |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 13 Feb 2019 13:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1977 |
Actions (login required)
 |
View Item |
