VerMilyea, Matthew D. and Maneck, Matthias and Yoshida, Naoko and Blochberger, Isabell and Suzuki, Emi and Suzuki, Toru and Spang, Rainer and Klein, Christoph A. and Perry, Anthony C. F. (2011) Transcriptome asymmetry within mouse zygotes but not between early embryonic sister blastomeres. EMBO JOURNAL, 30 (9). pp. 1841-1851. ISSN 0261-4189, 1460-2075
Full text not available from this repository. (Request a copy)Abstract
Transcriptome regionalization is an essential polarity determinant among metazoans, directing embryonic axis formation during normal development. Although conservation of this principle in mammals is assumed, recent evidence is conflicting and it is not known whether transcriptome asymmetries exist within unfertilized mammalian eggs or between the respective cleavage products of early embryonic divisions. We here address this by comparing transcriptome profiles of paired single cells and sub-cellular structures obtained microsurgically from mouse oocytes and totipotent embryos. Paired microsurgical spindle and remnant samples from unfertilized metaphase II oocytes possessed distinguishable profiles. Fertilization produces a totipotent 1-cell embryo (zygote) and associated spindle-enriched second polar body whose paired profiles also differed, reflecting spindle transcript enrichment. However, there was no programmed transcriptome asymmetry between sister cells within 2-or 3-cell embryos. Accordingly, there is transcriptome asymmetry within mouse oocytes, but not between the sister blastomeres of early embryos. This work places constraints on pre-patterning in mammals and provides documentation correlating potency changes and transcriptome partitioning at the single-cell level. The EMBO Journal (2011) 30, 1841-1851. doi:10.1038/emboj.2011.92; Published online 5 April 2011
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA LOCALIZATION; ORDERED GENE LISTS; XENOPUS OOCYTES; GENOME-WIDE; PREIMPLANTATION EMBRYOS; DROSOPHILA NEUROBLAST; EXPRESSION ANALYSIS; BLASTOCYST AXIS; VEGETAL CORTEX; SELF-RENEWAL; mouse metaphase II; polarity; pre-implantation embryo; pre-patterning; transcriptome |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Jun 2020 05:36 |
| Last Modified: | 22 Jun 2020 05:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/20838 |
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