Malfertheiner, S. Fill and Wendt, S. and Layer, F. and Weigl, M. and Seelbach-Goebel, B. and Koenig, W. and Koenig, B. (2017) Prevalence, clonality, and pathogenicity of Staphylococcus epidermidis isolates in newborn feces. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 36 (10). pp. 1955-1964. ISSN 0934-9723, 1435-4373
Full text not available from this repository. (Request a copy)Abstract
Coagulase-negative staphylococci (CoNS) are the most prevalent pathogens causing late-onset sepsis in neonates. The question is whether neonates acquire endemic hospital-adapted clones or incidentally occurring CoNS strains after birth during their hospital stay. Therefore, a prospective study was performed on the prevalence of CoNS in the stool of babies (born vaginally or by cesarean section) during their first days of life. Their clonal relatedness and potential to induce invasive disease were characterized. CoNS were analyzed from the stool samples of newborns with a load of CoNS above 10(3) colony-forming units (CFU)/mL. The identification of CoNS was performed phenotypically and genotypically. For typing, repetitive polymerase chain reaction (PCR), pulsed-field gel electrophoresis, and multilocus sequence typing were used. Resistance profiles, biofilm production, the presence of icaAD and of IS256 were determined as well. From a total of 207 stool samples (56 newborns), CoNS were detected in 41% of the newborns, mostly on day 3 for the first time (62.5%). Staphylococcus epidermidis was isolated in 85.7% of cases, harbored no IS256 element, and mostly expressed no biofilm. The isolates were separated into four main clusters by repetitive sequence-based PCR. 24% of the strains showed no antimicrobial resistance. 20% were resistant against four antibiotics of two different antibiotic classes. The remaining strains were resistant only against one antimicrobial substance class. Thus, it can be concluded that newborns do not acquire hospital-adapted endemic, multidrug-resistant S. epidermidis isolates during their first days of life. Yet, the results support the thesis that, during hospital stay, environmental parameters may convert sensible/noninvasive S. epidermidis strains into multidrug-resistant strains with characteristics of invasiveness.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COAGULASE-NEGATIVE STAPHYLOCOCCI; BIRTH-WEIGHT INFANTS; INTENSIVE-CARE-UNIT; MOLECULAR EPIDEMIOLOGY; BIOFILM FORMATION; JOINT PROSTHESES; AUREUS STRAINS; INFECTIONS; IDENTIFICATION; FLORA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Geburtshilfe) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 19 Feb 2019 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2142 |
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