Hlavacek, Antonin and Peterek, Miroslav and Farka, Zdenek and Mickert, Matthias J. and Prechtl, Leonhard and Knopp, Dietmar and Gorris, Hans H. (2017) Rapid single-step upconversion-linked immunosorbent assay for diclofenac. MICROCHIMICA ACTA, 184 (10). pp. 4159-4165. ISSN 0026-3672, 1436-5073
Full text not available from this repository. (Request a copy)Abstract
The non-steroidal anti-inflammatory drug and analgesic diclofenac is a common micropollutant in water. A direct competitive upconversion-linked immunosorbent assay (ULISA) for diclofenac has been developed that is based on a nanoparticulate tracer consisting of a NaYF4:Yb, Er core (diameter of similar to 90 nm, excitation and emission wavelenghts 980 and 535 nm, respectively) enclosed by a carboxylated silica shell and finally coated with diclofenac-conjugated bovine gamma-globulin. The proteinaceous coating prevents non-specific adsorption of the tracer to the microtiter plate and provides a structurally flexible linker for surface-exposed diclofenac to warrant efficient competition with free (analyte) diclofenac in real water samples. The tracer was purified by gel electrophoresis and lyophilized. Both processes have no adverse effects on the immunoassay. All assay components can be stored in a dry state without a cooling chain, and can be reactivated on demand. Hence, this ULISA is well suited for environmental monitoring in low-resource settings. The ULISA has a similar limit of detection (20 pg mL(-1); equivalent to 70 pM) as the conventional ELISA, but the time for analysis is reduced to 70 min because no enzymatic amplification steps are involved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTON-UPCONVERTING NANOPARTICLES; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; PHARMACEUTICAL RESIDUES; ENVIRONMENTAL-SAMPLES; AQUATIC ENVIRONMENT; CARBON NANOTUBES; WATER; SURFACE; PHASE; Bioconjugation; Electrophoretic purification; Immunoassay; Luminescence; Pharmaceutical micropollutant; Photon-upconversion |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:19 |
| Last Modified: | 13 Feb 2019 14:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2144 |
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