Protti, Andrea and Mongue-Din, Heloise and Mylonas, Katie J. and Sirker, Alexander and Sag, Can Martin and Swim, Megan M. and Maier, Lars and Sawyer, Greta and Dong, Xuebin and Botnar, Rene and Salisbury, Jon and Gray, Gillian A. and Shah, Ajay M. (2016) Bone marrow transplantation modulates tissue macrophage phenotype and enhances cardiac recovery after subsequent acute myocardial infarction. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 90. pp. 120-128. ISSN 0022-2828, 1095-8584
Full text not available from this repository. (Request a copy)Abstract
Background: Bone marrow transplantation (BMT) is commonly used in experimental studies to investigate the contribution of BM-derived circulating cells to different disease processes. During studies investigating the cardiac response to acute myocardial infarction (MI) induced by permanent coronary ligation in mice that had previously undergone BMT, we found that BMT itself affects the remodelling response. Methods and results: Compared to matched naive mice, animals that had previously undergone BMT developed significantly less post-MI adverse remodelling, infarct thinning and contractile dysfunction as assessed by serial magnetic resonance imaging. Cardiac rupture in male mice was prevented. Histological analysis showed that the infarcts of mice that had undergone BMT had a significantly higher number of inflammatory cells, surviving cardiomyocytes and neovessels than control mice, as well as evidence of significant haemosiderin deposition. Flow cytometric and histological analyses demonstrated a higher number of alternatively activated (M2) macrophages in myocardium of the BMT group compared to control animals even before MI, and this increased further in the infarcts of the BMT mice after MI. Conclusions: The process of BMT itself substantially alters tissue macrophage phenotype and the subsequent response to acute MI. An increase in alternatively activated macrophages in this setting appears to enhance cardiac recovery after MI. (C) 2015 The Authors. Published by Elsevier Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOAM CELL-FORMATION; ISCHEMIC-MYOCARDIUM; HEME OXYGENASE-1; PROGENITOR CELLS; APOPTOTIC CELLS; MICE; REPERFUSION; HEMORRHAGE; INJURY; ATHEROSCLEROSIS; Bone marrow transplantation; Macrophage polarisation; Myocardial infarction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2019 12:36 |
| Last Modified: | 06 Mar 2019 08:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2177 |
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