Merkl, Rainer and Sterner, Reinhard (2016) Ancestral protein reconstruction: techniques and applications. BIOLOGICAL CHEMISTRY, 397 (1). pp. 1-21. ISSN 1431-6730, 1437-4315
Full text not available from this repository. (Request a copy)Abstract
Ancestral sequence reconstruction (ASR) is the calculation of ancient protein sequences on the basis of extant ones. It is most powerful in combination with the experimental characterization of the corresponding proteins. Such analyses allow for the study of problems that are otherwise intractable. For example, ASR has been used to characterize ancestral enzymes dating back to the Paleoarchean era and to deduce properties of the corresponding habitats. In addition, the historical approach underlying ASR enables the identification of amino acid residues key to protein function, which is often not possible by only comparing extant proteins. Along these lines, residues responsible for the spectroscopic properties of protein pigments were identified as well as residues determining the binding specificity of steroid receptors. Further applications are studies related to the longevity of mutations, the contribution of gene duplications to enzyme functionalization, and the evolution of protein complexes. For these applications of ASR, we discuss recent examples; moreover, we introduce the basic principles of the underlying algorithms and present state-of-the-art protocols.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAXIMUM-LIKELIHOOD APPROACH; SPECIES TREE ESTIMATION; AMINO-ACID-SEQUENCES; PHYLOGENETIC INFERENCE; DNA-SEQUENCES; GENE TREES; EVOLUTIONARY HISTORY; RESURRECTED PROTEINS; NUCLEOTIDE-SEQUENCES; SUBSTITUTION MODELS; ancestral sequence reconstruction; phylogenetic analysis; protein evolution; vertical analysis of protein function |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl |
| Depositing User: | Petra Gürster |
| Date Deposited: | 01 Mar 2019 12:36 |
| Last Modified: | 03 Sep 2020 09:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2258 |
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