Minuth, Will W. and Denk, Lucia (2016) Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system. CYTOTECHNOLOGY, 68 (2). pp. 179-196. ISSN 0920-9069, 1573-0778
Full text not available from this repository. (Request a copy)Abstract
To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEETA (R) tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENTIN BARRIER TEST; IN-VITRO MODEL; TEXTILE CHITOSAN SCAFFOLDS; MARROW STROMAL CELLS; LONG-TERM CULTURE; ELECTROSPUN POLYMER SCAFFOLDS; DEPENDENT NEURONAL PLASTICITY; TISSUE-ENGINEERED CARTILAGE; RETINAL-PIGMENT EPITHELIUM; COLLECTING DUCT EPITHELIA; Cell culture; Perfusion culture; 3D culture; Tissue carrier; Bioreactor; Tissue engineering; Biomaterial testing; Biomedicine |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Petra Gürster |
| Date Deposited: | 03 Sep 2020 06:53 |
| Last Modified: | 03 Sep 2020 06:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2364 |
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