Hillenkamp, Jost and Dydykina, Sofia and Klettner, Alexa and Treumer, Felix and Vasold, Rudolf and Baeumler, Wolfgang and Roider, Johann (2010) SAFETY TESTING OF INDOCYANINE GREEN WITH DIFFERENT SURGICAL LIGHT SOURCES AND THE PROTECTIVE EFFECT OF OPTICAL FILTERS. RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES, 30 (10). pp. 1685-1691. ISSN 0275-004X,
Full text not available from this repository. (Request a copy)Abstract
Purpose: The purpose of this study was to assess the light-induced cytotoxicity of indocyanine green (ICG) using different light sources commonly used in macular surgery and to assess the effect of optical filters. Methods: Primary cultures of porcine retinal pigment epithelium cells were incubated with 0.5 mg/mL ICG solution dissolved in 5% glucose and illuminated with a surgical light fiber for 3 or 15 minutes. Halogen, mercury vapor, xenon, and metal halide light sources were used. Cell viability was assessed using the MTT assay. Retinal pigment epithelium cells without illumination served as controls. The decomposition of ICG after illumination was analyzed by high-performance liquid chromatography. Results: Illumination of retinal pigment epithelium cells with all light sources with or without previous incubation with ICG did not affect cell viability compared with controls. Cell viability was significantly reduced when the cells were not rinsed immediately after incubation. The cytotoxic effect was abolished by a 475-nm long-pass filter. The high-performance liquid chromatography analysis of the illuminated ICG solution identified six cytotoxic ICG decomposition products. Conclusion: Optical filters that narrow the emission spectrum of the light sources reduce the light-induced cytotoxicity of ICG. Optical filters applied in ICG-assisted macular surgery may reduce the risk of intraoperative cell damage. RETINA 30:1685-1691, 2010
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERNAL LIMITING MEMBRANE; MACULAR HOLE SURGERY; RETINAL-PIGMENT EPITHELIUM; IDIOPATHIC EPIRETINAL MEMBRANE; BRILLIANT-BLUE-G; IN-VITRO; VITAL DYES; VITRECTOMY; REMOVAL; CELLS; endoillumination; phototoxicity; surgical light source; vitrectomy; indocyanine green |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 660 Chemical engineering |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jul 2020 05:22 |
| Last Modified: | 08 Jul 2020 05:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/23984 |
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