Tyagi, A. and Penzkofer, Alfons and Mathes, T. and Hegemann, P. (2010) Photophysical characterisation and photo-cycle dynamics of LOV1-His domain of phototropin from Chlamydomonas reinhardtii with roseoflavin monophosphate cofactor. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 101 (1). pp. 76-88. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
The wild-type phototropin protein phot from the green alga Chlamydomonas reinhardtii with the blue-light photoreceptor domains LOV1 and LOV2 has flavin mononucleotide (FMN) as cofactor. For the LOV1-His domain from phot of C. reinhardtii studied here, the FMN chromophore was replaced by roseoflavin monophosphate (8-dimethylamino-8-demethyl-FMN, RoFMN) during heterologous expression in a riboflavin auxotropic Escherichia colt strain. An absorption and emission spectroscopic characterisation of the cofactor exchanged-LOV1-His (RoLOV1) domain was carried out in aqueous pH 8 phosphate buffer. The fluorescence of RoLOV1 is quenched by photo-induced charge transfer at room temperature. The photo-cyclic dynamics of RoLOV1 was observed by blue-light induced hypochromic and bathochromic absorption changes which recover on a minute timescale in the dark. Photo-excited RoFMN is thought to cause reversible protein and cofactor structural changes. Prolonged intense blue-light exposure caused photo-degradation of RoFMN in RoLOV1 to fully reduced flavin and lumichrome derivatives. Photo-cycle schemes of RoLOV1 and LOV1 are presented, and the photo-degradation dynamics of R0LOV1 is discussed. (C) 2010 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLUE-LIGHT RECEPTOR; EMISSION SPECTROSCOPIC CHARACTERIZATION; INTRAMOLECULAR CHARGE-TRANSFER; SEXUAL LIFE-CYCLE; RHODOBACTER-SPHAEROIDES; ELECTRON-TRANSFER; LOV2 DOMAIN; SIGNAL-TRANSDUCTION; RIBOFLAVIN ANALOGS; ABSORPTION-SPECTRA; Roseoflavin monophosphate; Phototropin; Cofactor-exchanged LOV1 domain; RoLOV photo-cycle; LOV photo-cycle; Photo-induced protein re-conformation |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jul 2020 08:51 |
| Last Modified: | 08 Jul 2020 08:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/24020 |
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