Vijayakumar, Vinod and Liebisch, Gerhard and Buer, Benjamin and Xue, Li and Gerlach, Nina and Blau, Samira and Schmitz, Jessica and Bucher, Marcel (2016) Integrated multi-omics analysis supports role of lysophosphatidylcholine and related glycerophospholipids in the Lotus japonicus-Glomus intraradices mycorrhizal symbiosis. PLANT CELL AND ENVIRONMENT, 39 (2). pp. 393-415. ISSN 0140-7791, 1365-3040
Full text not available from this repository. (Request a copy)Abstract
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus, we performed an integrated multi-omics analysis with a focus on plant and fungal phospholipid (PL) metabolism and biological significance of lysophosphatidylcholine (LPC). Our results support the role of LPC as a bioactive compound eliciting cellular and molecular response mechanisms in Lotus. Evidence is provided for large interspecific chemical diversity of LPC species among mycorrhizae with related AMF species. Lipid, gene expression and elemental profiling emphasize the Lotus-Glomus intraradices interaction as distinct from other arbuscular mycorrhizal (AM) interactions. In G. intraradices, genes involved in fatty acid (FA) elongation and biosynthesis of unsaturated FAs were enhanced, while in Lotus, FA synthesis genes were up-regulated during AMS. Furthermore, FAS protein localization to mitochondria suggests FA biosynthesis and elongation may also occur in AMF. Our results suggest the existence of interspecific partitioning of PL resources for generation of LPC and novel candidate bioactive PLs in the Lotus-G. intraradices symbiosis. Moreover, the data advocate research with phylogenetically diverse Glomeromycota species for a broader understanding of the molecular underpinnings of AMS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FATTY-ACID SYNTHESIS; HIGH-THROUGHPUT QUANTIFICATION; TANDEM MASS-SPECTROMETRY; MEDICAGO-TRUNCATULA; ARBUSCULAR MYCORRHIZA; SACCHAROMYCES-CEREVISIAE; TRANSGENIC PLANTS; H+-ATPASE; FUNCTIONAL-CHARACTERIZATION; PHOSPHATE TRANSPORTER; Glomus intraradices (syn: Rhizophagus irregularis); arbuscular mycorrhiza; fatty acid synthesis; Glomeromycota; ionomics; lipidomics; phospholipids; transcriptomics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Mar 2019 07:19 |
| Last Modified: | 14 Mar 2019 07:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2468 |
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