Hummel, Irena Brunskole and Zitzmann, Anita and Erl, Monika and Wenzel, Juergen J. and Jilg, Wolfgang (2016) Characteristics of immune memory 10-15 years after primary hepatitis B vaccination. VACCINE, 34 (5). pp. 636-642. ISSN 0264-410X, 1873-2518
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Background and aims: The definition of immune memory after hepatitis B vaccination is still under debate. Therefore, we analysed hepatitis B surface antigen (HBsAg)-specific memory in more detail by investigating the kinetics of humoral and cellular responses after hepatitis B booster vaccination. Methods: The anti-HBs kinetics of 23 individuals with anti-HBs titres below 10 IU/I, who had been vaccinated 10-15 years ago, was monitored at day 0, 3, 7,14 and 28 after booster vaccination. HBsAg-specific IFN gamma- and IL5-secreting cells in enriched CD4(+) fraction were measured at day 0, 7 and 28 post-booster by enzyme-linked immunospot assay (ELISpot). Results: 22 of 23 subjects showed similar anti-HBs kinetic curves, including 3 of 4 subjects who did not reach anti-HBs titres of 10 IU/I. The steep anti-HBs increase started between day 3 and 7 and peaked around day 14. A plateau or only minimal changes were visible between day 14 and 28. 17.4% of subjects showed pre-booster cellular responses, and this rate had increased to 47.8% and 56.5% after 7 and 28 days, respectively. The kinetic patterns of T cell responses differed considerably among subjects. A dominance of Th2 responses (IL5 secretion) over Th1 responses (IFN gamma secretion) could be observed. Conclusions: The presence of B cell memory could be shown by a typical anamnestic anti-HBs response curve after a booster dose in all but one individual. In contrast, T cell responses to booster vaccination, which occurred in approximately 50% of participants, were rather heterogeneous. (C) 2015 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEALTH-CARE WORKERS; SURFACE-ANTIGEN; ANTIBODY-RESPONSE; T-CELL; NEONATAL IMMUNIZATION; INTERFERON-GAMMA; BOOSTER; VIRUS; ADOLESCENTS; PROTECTION; Hepatitis B vaccination; Immune memory; Kinetics; Anti-HBs titre; Cellular immunity; ELISpot |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2019 07:24 |
| Last Modified: | 15 Mar 2019 07:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2526 |
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