Walz, Johanna M. and Boehringer, Daniel and Deissler, Heidrun L. and Faerber, Lothar and Goepfert, Jens C. and Heiduschka, Peter and Kleeberger, Susannah M. and Klettner, Alexa and Krohne, Tim U. and Schneiderhan-Marra, Nicole and Ziemssen, Focke and Stahl, Andreas (2016) Pre-Analytical Parameters Affecting Vascular Endothelial Growth Factor Measurement in Plasma: Identifying Confounders. PLOS ONE, 11 (1): e0145375. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Background Vascular endothelial growth factor-A (VEGF-A) is intensively investigated in various medical fields. However, comparing VEGF-A measurements is difficult because sample acquisition and pre-analytic procedures differ between studies. We therefore investigated which variables act as confounders of VEGF-A measurements. Methods Following a standardized protocol, blood was taken at three clinical sites from six healthy participants (one male and one female participant at each center) twice one week apart. The following pre-analytical parameters were varied in order to analyze their impact on VEGF-A measurements: analyzing center, anticoagulant (EDTA vs. PECT/CTAD), cannula (butterfly vs. neonatal), type of centrifuge (swing-out vs. fixed-angle), time before and after centrifugation, filling level (completely filled vs. half-filled tubes) and analyzing method (ELISA vs. multiplex bead array). Additionally, intrapersonal variations over time and sex differences were explored. Statistical analysis was performed using a linear regression model. Results The following parameters were identified as statistically significant independent confounders of VEGF-A measurements: analyzing center, anticoagulant, centrifuge, analyzing method and sex of the proband. The following parameters were no significant confounders in our data set: intrapersonal variation over one week, cannula, time before and after centrifugation and filling level of collection tubes. Conclusion VEGF-A measurement results can be affected significantly by the identified pre-analytical parameters. We recommend the use of CTAD anticoagulant, a standardized type of centrifuge and one central laboratory using the same analyzing method for all samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METASTATIC COLORECTAL-CANCER; MACULAR DEGENERATION; INTRAVITREAL INJECTION; SERUM CONCENTRATIONS; RANDOMIZED-TRIAL; DOUBLE-BLIND; BEVACIZUMAB; VEGF; ANGIOGENESIS; RANIBIZUMAB; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehreinheit Pharmakologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2019 08:28 |
| Last Modified: | 15 Mar 2019 08:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2556 |
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