De Simone, Gabriele and Mazza, Emilia M. C. and Cassotta, Antonino and Davydov, Alexey N. and Kuka, Mirela and Zanon, Veronica and De Paoli, Federica and Scamardella, Eloise and Metsger, Maria and Roberto, Alessandra and Pilipow, Karolina and Colombo, Federico S. and Tenedini, Elena and Tagliafico, Enrico and Gattinoni, Luca and Mavilio, Domenico and Peano, Clelia and Price, David A. and Singh, Satya P. and Farber, Joshua M. and Serra, Valentina and Cucca, Francesco and Ferrari, Francesco and Orru, Valeria and Fiorillo, Edoardo and Iannacone, Matteo and Chudakov, Dmitriy M. and Sallusto, Federica and Lugli, Enrico (2019) CXCR3 Identifies Human Naive CD8(+) T Cells with Enhanced Effector Differentiation Potential. JOURNAL OF IMMUNOLOGY, 203 (12). pp. 3179-3189. ISSN 0022-1767, 1550-6606
Full text not available from this repository. (Request a copy)Abstract
In mice, the ability of naive T (T-N) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8(+) T-N cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3(+) T-N cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3(+) T-N cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3(+) T-N cells were transcriptionally equivalent to murine CXCR3(+) T-N cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8(+) T cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR EXPRESSION; FLOW-CYTOMETRY; CENTRAL MEMORY; CD4(+); REPERTOIRE; PHENOTYPE; EXPANSION; RECONSTITUTION; POPULATIONS; DIVERSITY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Mar 2020 07:21 |
| Last Modified: | 20 Mar 2020 07:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25673 |
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