Mandlik, V. and Kehrer, A. and Jiga, L. and Hoinoiu, B. and Ionac, M. and Jung, F. and Staudenmaier, R. and Prantl, L. (2016) Prefabrication and free transfer of a tissue engineered composite flap - An experimental model in the rat. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 64 (3). pp. 319-331. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: The technique of flap-prefabrication has been successfully established in tissue engineering: missing intrinsic vascularisation of engineered tissue can be generated in vivo by microsurgical vesselloop construction. It is possible to move engineered tissue into a defect with microsurgery. In the literature, the combination of engineered tissue covered with skin is not widely reported. OBJECTIVE: Aim of this study was to establish a model to investigate scaffold prefabrication with full thickness skin graft coverage with subsequent free tissue transfer. METHODS: 8Wistar rats were operated in 2 separate steps: 1) after creating an arteriovenous loop with the femoral vessels, a porous scaffold was placed on the loop and covered with an inguinally based skin flap. A control was implanted without loop into the contralateral groin. 2) 6 weeks later the prefabricated composite flaps were microsurgically transferred to the cervical region. Skin-island monitoring was performed with Laser Doppler-scanner after the transfer. RESULTS: Continuous loss of the skin islands was observed within 72 hours. Complications included wound-dehiscence, thrombosis and death from anaesthesia; in spite of consistent loop viability. CONCLUSION: Evaluation showed that modifications are necessary to maintain the skin-island cove.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKIN FLAPS; VASCULAR CARRIER; RECONSTRUCTION; CARTILAGE; RABBITS; PRELAMINATION; ANGIOGENESIS; BUNDLE; Composite tissue; tissue engineering applications; animal models; enabling technologies; angiogenesis and vasculogenesis; fundamentals of tissue engineering |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Mar 2019 09:52 |
| Last Modified: | 13 Mar 2019 09:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2577 |
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