Pauly, Diana and Agarwal, Divyansh and Dana, Nicholas and Schaefer, Nicole and Biber, Josef and Wunderlich, Kirsten A. and Jabri, Yassin and Straub, Tobias and Zhang, Nancy R. and Gautam, Avneesh K. and Weber, Bernhard H. F. and Hauck, Stefanie M. and Kim, Mijin and Curcio, Christine A. and Stambolian, Dwight and Li, Mingyao and Grosche, Antje (2019) Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina. CELL REPORTS, 29 (9). 2835-+. ISSN 2211-1247,
Full text not available from this repository. (Request a copy)Abstract
Complement dysregulation is a feature of many retinal diseases, yet mechanistic understanding at the cellular level is limited. Given this knowledge gap about which retinal cells express complement, we performed single-cell RNA sequencing on similar to 92,000 mouse retinal cells and validated our results in five major purified retinal cell types. We found evidence for a distributed cell-type-specific complement expression across 11 cell types. Notably, Muller cells are the major contributor of complement activators c1s, c3, c4, and cfb. Retinal pigment epithelium (RPE) mainly expresses cfh and the terminal complement components, whereas cfi and cfp transcripts are most abundant in neurons. Aging enhances c1s, cfb, cfp, and cfi expression, while cfh expression decreases. Transient retinal ischemia increases complement expression in microglia, Muller cells, and RPE. In summary, we report a unique complement expression signature for murine retinal cell types suggesting a well-orchestrated regulation of local complement expression in the retinal microenvironment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GENE-EXPRESSION; PIGMENT EPITHELIUM; GLIAL-CELLS; MOUSE MODEL; FACTOR-I; ACTIVATION; SYSTEM; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Mar 2020 07:09 |
| Last Modified: | 25 Mar 2020 07:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/25800 |
Actions (login required)
 |
View Item |
