Damman, Reinier and Schuetz, Stefan and Luo, Yanzhang and Weingarth, Markus and Sprangers, Remco and Baldus, Marc (2019) Atomic-level insight into mRNA processing bodies by combining solid and solution-state NMR spectroscopy. NATURE COMMUNICATIONS, 10: 4536. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Liquid-liquid phase separation is increasingly recognized as a process involved in cellular organization. Thus far, a detailed structural characterization of this intrinsically heterogeneous process has been challenging. Here we combine solid- and solution-state NMR spectroscopy to obtain atomic-level insights into the assembly and maturation of cytoplasmic processing bodies that contain mRNA as well as enzymes involved in mRNA degradation. In detail, we have studied the enhancer of decapping 3 (Edc3) protein that is a central hub for processing body formation in yeast. Our results reveal that Edc3 domains exhibit diverse levels of structural organization and dynamics after liquid-liquid phase separation. In addition, we find that interactions between the different Edc3 domains and between Edc3 and RNA in solution are largely preserved in the condensed protein state, allowing processing bodies to rapidly form and dissociate upon small alterations in the cellular environment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHASE-SEPARATION; PROTEIN; EFFICIENT; HYDROGEL; LIQUID; DOMAIN; DROPLETS; DYNAMICS; PROMOTES; FIBRILS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Remco Sprangers |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Mar 2020 05:44 |
| Last Modified: | 01 Apr 2020 05:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26025 |
Actions (login required)
 |
View Item |
