Kramm, Kevin and Endesfelder, Ulrike and Grohmann, Dina (2019) A Single-Molecule View of Archaeal Transcription. JOURNAL OF MOLECULAR BIOLOGY, 431 (20). pp. 4116-4131. ISSN 0022-2836, 1089-8638
Full text not available from this repository. (Request a copy)Abstract
The discovery of the archaeal domain of life is tightly connected to an in-depth analysis of the prokaryotic RNA world. In addition to Carl Woese's approach to use the sequence of the 16S rRNA gene as phylogenetic marker, the finding of Karl Stetter and Wolfram Zillig that archaeal RNA polymerases (RNAPs) were nothing like the bacterial RNAP but are more complex enzymes that resemble the eukaryotic RNAPII was one of the key findings supporting the idea that archaea constitute the third major branch on the tree of life. This breakthrough in transcriptional research 40 years ago paved the way for in-depth studies of the transcription machinery in archaea. However, although the archaeal RNAP and the basal transcription factors that fine-tune the activity of the RNAP during the transcription cycle are long known, we still lack information concerning the architecture and dynamics of archaeal transcription complexes. In this context, single-molecule measurements were instrumental as they provided crucial insights into the process of transcription initiation, the architecture of the initiation complex and the dynamics of mobile elements of the RNAP. In this review, we discuss single-molecule approaches suitable to examine molecular mechanisms of transcription and highlight findings that shaped our understanding of the archaeal transcription apparatus. We furthermore explore the possibilities and challenges of next-generation single-molecule techniques, for example, super-resolution microscopy and single-molecule tracking, and ask whether these approaches will ultimately allow us to investigate archaeal transcription in vivo. (C) 2019 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-POLYMERASE-II; TATA-BINDING PROTEIN; NUCLEOID-ASSOCIATED PROTEIN; NANO-POSITIONING SYSTEM; LIVE ESCHERICHIA-COLI; RAY CRYSTAL-STRUCTURE; STRUCTURAL BASIS; SPATIAL-ORGANIZATION; ABORTIVE INITIATION; GENE-REGULATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Petra Gürster |
| Date Deposited: | 06 Apr 2020 08:44 |
| Last Modified: | 06 Apr 2020 08:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26231 |
Actions (login required)
 |
View Item |
