Pfeifer, Christian G. and Karl, Alexandra and Berner, Arne and Zellner, Johannes and Schmitz, Paul and Loibl, Markus and Koch, Matthias and Angele, Peter and Nerlich, Michael and Mueller, Michael B. (2016) Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs. STEM CELLS INTERNATIONAL: 2685147. ISSN 1687-966X, 1687-9678
Full text not available from this repository. (Request a copy)Abstract
Chondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-beta (TGF-beta) and BMP signalling during chondrogenesis. Both TGF-beta and BMP signalling are regulators of chondrogenic cell differentiation. Human bone marrow derived MSCs were chondrogenically predifferentiated in aggregate culture for 14 days. Thereafter, one group was subjected to hypertrophy enhancing media conditions while controls were kept in chondrogenic medium until day 28. Histological evaluation, gene expression by PCR, and Western blot analysis were carried out at days 1, 3, 7, 14, 17, 21, and 28. A subset of cultures was treated with the BMP inhibitor Noggin to test for BMP dependent expression of BAMBI. Hypertrophic differentiated pellets showed larger cells with increased collagen 10 and alkaline phosphatase staining. There was significantly increased expression of BAMBI on gene expression and protein level in hypertrophic cultures compared to the chondrogenic control and increased BMP4 gene expression. Immunohistochemistry showed intense staining of BAMBI in hypertrophic cells. BAMBI expression was dose-dependently downregulated by Noggin. The pseudoreceptor BAMBI is upregulated upon enhancement of hypertrophy in MSC chondrogenic differentiation by a BMP dependent mechanism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; VITRO CARTILAGE FORMATION; BONE-MARROW; CHONDROGENIC DIFFERENTIATION; TRANSCRIPTIONAL REGULATION; PSEUDORECEPTOR BAMBI; ENGINEERED CARTILAGE; GENE-EXPRESSION; BETA; HYPERTROPHY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Mar 2019 06:37 |
| Last Modified: | 14 Mar 2019 06:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2625 |
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