Garcia-Descalzo, Laura and Parro, Victorino and Garcia-Villadangos, Miriam and Cockell, Charles S. and Moissl-Eichinger, Christine and Perras, Alex and Rettberg, Petra and Beblo-Vranesevic, Kristina and Bohmeier, Maria and Rabbow, Elke and Westall, Frances and Gaboyer, Frederik and Amils, Ricardo and Malki, Moustafa and Marteinsson, Viggo and Vannier, Pauline and Ehrenfreund, Pascale and Monaghan, Euan and Riedo, Andreas and Cabezas, Patricia and Walter, Nicolas and Gomez Gomez, Felipe (2019) Microbial Markers Profile in Anaerobic Mars Analogue Environments Using the LDChip (Life Detector Chip) Antibody Microarray Core of the SOLID (Signs of Life Detector) Platform. MICROORGANISMS, 7 (9): 365. ISSN , 2076-2607
Full text not available from this repository. (Request a copy)Abstract
One of the main objectives for astrobiology is to unravel and explore the habitability of environments beyond Earth, paying special attention to Mars. If the combined environmental stress factors on Mars are compatible with life or if they were less harsh in the past, to investigate the traces of past or present life is critical to understand its potential habitability. Essential for this research is the characterization of Mars analogue environments on Earth through the development of techniques for biomarker detection in them. Biosensing techniques based on fluorescence sandwich microarray immunoassays (FSMI) have shown to be a powerful tool to detect biosignatures and depict the microbial profiles of different environments. In this study, we described the microbial biomarker profile of five anoxic Mars analogues sites using the Life Detector Chip (LDChip), an antibody microarray for multiple microbial marker detection. Furthermore, we contributed to new targets by developing a new 26-polyclonal antibodies microarray using crude extracts from anaerobic sampling sites, halophilic microorganisms, and anaerobic isolates obtained in the framework of the European Mars Analogues for Space Exploration (MASE) project. The new subset of antibodies was characterized and implemented into a microarray platform (MASE-Chip) for microbial marker searching in salty and anaerobic environments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECURRING SLOPE LINEAE; CHEMICAL-COMPOSITION; PROPIDIUM MONOAZIDE; NATURAL COMMUNITIES; WATER ACTIVITY; LIQUID WATER; SUBSURFACE; SURFACE; HABITABILITY; BIOMARKERS; anaerobic environments; Mars; analogues; biomarkers; microarray |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 09:58 |
| Last Modified: | 06 Apr 2020 09:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26295 |
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