Vatsyayan, Preety and Iffelsberger, Christian and Mayorga-Martinez, Carmen C. and Matysik, Frank-Michael (2016) Imaging of localized enzymatic peroxidase activity over unbiased individual gold nanowires by scanning electrochemical microscopy. ANALYTICAL METHODS, 8 (38). pp. 6847-6855. ISSN 1759-9660, 1759-9679
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Scanning electrochemical microscopy (SECM) in the constant-height mode was used to image individual gold nanowires (AuNWs, 2-3 mu m long and similar to 140 nm diameter). High-resolution negative and positive feedback current images of individual AuNWs immobilized on glass and gold-coated glass slides, respectively, were recorded with a Wollaston-based platinum disk ultramicroelectrode (UME) of radius 300 nm at 0.3 V probe potential using ferrocenemethanol as the mediator. The negative and positive feedback current responses were dependent on the effective recycling of the mediator on the unbiased AuNWs. Furthermore, the AuNWs were covalently linked to horseradish peroxidase (HRP) and immobilized on a thin film gold substrate. Positive feedback and localized HRP activity over individual AuNWs were imaged subsequently by switching the probe potential from 0.3 V to 0 V after the addition of H2O2. In this measurement, ferrocenemethanol behaved both as the mediator (feedback response at 0.3 V probe potential) and the electron donor for HRP (in the presence of H2O2 for activity imaging at 0 V).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HETEROGENEOUS ELECTRON-TRANSFER; WALLED CARBON NANOTUBES; AU NANOWIRES; NANOPARTICLES; BIOSENSORS; SECM; CELLS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2019 11:02 |
| Last Modified: | 18 Mar 2019 11:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2631 |
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