Baumstark, Daniela and Kremer, Werner and Boettcher, Alfred and Schreier, Christina and Sander, Paul and Schmitz, Gerd and Kirchhoefer, Renate and Huber, Fritz and Kalbitzer, Hans Robert (2019) H-1 NMR spectroscopy quantifies visibility of lipoproteins, subclasses, and lipids at varied temperatures and pressures[S]. JOURNAL OF LIPID RESEARCH, 60 (9). pp. 1516-1534. ISSN 0022-2275, 1539-7262
Full text not available from this repository. (Request a copy)Abstract
NMR-based quantification of human lipoprotein (sub)classes is a powerful high-throughput method for medical diagnostics. We evaluated select proton NMR signals of serum lipoproteins for elucidating the physicochemical features and the absolute NMR visibility of their lipids. We separated human lipoproteins of different subclasses by ultracentrifugation and analyzed them by H-1 NMR spectroscopy at different temperatures (283-323 K) and pressures (0.1-200 MPa). In parallel, we determined the total lipid content by extraction with chloroform/methanol. The visibility of different lipids in the H-1 NMR spectra strongly depends on temperature and pressure: it increases with increasing temperatures but decreases with increasing pressures. Even at 313 K, only part of the lipoprotein is detected quantitatively. In LDL and in HDL subclasses HDL2 and HDL3, only 39%, 62%, and 90% of the total cholesterol and only 73%, 70%, and 87% of the FAs are detected, respectively. The choline head groups show visibilities of 43%, 75%, and 87% for LDL, HDL2, and HDL3, respectively. The description of the NMR visibility of lipid signals requires a minimum model of three different compartments, A, B, and C. The thermodynamic analysis of compartment B leads to melting temperatures between 282 K and 308 K and to enthalpy differences that vary for the different lipoproteins as well as for the reporter groups selected. In summary, we describe differences in NMR visibility of lipoproteins and variations in biophysical responses of functional groups that are crucial for the accuracy of absolute NMR quantification.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; LOW-DENSITY-LIPOPROTEIN; NEURAL-NETWORK ANALYSIS; HUMAN-PLASMA; PROTON NMR; CHEMICAL-SHIFTS; THERMAL TRANSITIONS; CHOLESTERYL ESTERS; CORE; APOLIPOPROTEIN; metabolomics; serum lipids; intermediate density lipoprotein; very low density lipoprotein; low density lipoprotein; high density lipoprotein; high pressure nuclear magnetic resonance |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2020 08:23 |
| Last Modified: | 06 Apr 2020 06:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26349 |
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